Table 1 List of primers and PCR amplification conditions used for VSX1 gene amplification
From: Analysis of the VSX1 gene in sporadic keratoconus patients from China
Exon | Sequence of primers (5′-3′) | Annealing temperature (°C) | Product length (bp) |
|---|---|---|---|
1 | CAGCTGATTGGAGCCCTTC(sense) | 62 | 599 |
CTCAGAGCCTAGGGGACAGG(antisense) | |||
2 | CTTAAGTACCCAAGAGGTTCATAACT(sense) | 62 | 269 |
GAAACCACTGGGCCTGCTATCAT(antisense) | |||
3 | AAGCAGGCACGGTGGTCCTTA(sense) | 63 | 293 |
AAGGGACTGCTGATTGGCTCACT(antisense) | |||
4 | GCTCGGGAGAGAAGATCC(sense) | 62 | 399 |
TCAGTAAACTGACGTTGCTTG(antisense) | |||
5 | GGAAATTTACTTCATTGCTGAATTT(sense) | 60 | 495 |
TGGCATTGCATTTTATCTTGACA(antisense) |