TLR4 modulates inflammatory gene targets in the retina during Bacillus cereus endophthalmitis

BMC Ophthalmology

Table 1 Microarray analysis of retinal genes upregulated 5-fold and higher 4 h postinfection with B. cereus ATCC14579

Gene symbol	Gene title	RefSeq Transcript ID	Fold-Change (C57BL6/J infected versus uninfected)	p-value	Fold-Change (TLR4^−/− infected versus uninfected)	p-value
CXCL1	chemokine (C-X-C motif) ligand 1	NM_008176	34	0.0106	NC	NS
CXCL2	chemokine (C-X-C motif) ligand 2	NM_009140	29	0.0225	NC	NS
IL-6	interleukin 6	NM_031168	25	0.0114	NC	NS
CXCL10	chemokine (C-X-C motif) ligand 10	NM_021274	21	0.0328	NC	NS
CCL2	chemokine (C-C motif) ligand 2	NM_011333	20	0.0355	NC	NS
CCL3	chemokine (C-C motif) ligand 3	NM_011337	16	0.0006	5	0.0446
PTX3	pentraxin related gene	NM_008987	15	0.0376	NC	NS
ICAM1	intercellular adhesion molecule 1	NM_010493	11	0.0026	NC	NS
SOCS3	suppressor of cytokine signaling 3	NM_007707	10	0.0034	NC	NS
CYR61	cysteine rich protein 61	NM_010516	10	0.0116	NC	NS
MOBP	myelin-associated oligodendrocytic basic protein	NM_008614	NC	NS	10	0.0270
MBP	myelin basic protein	NM_001025245	NC	NS	10	0.0223
PTGS2	prostaglandin-endoperoxide synthase 2	NM_011198	9	0.0027	NC	NS
STEAP4	STEAP family member 4	NM_054098	6	0.0116	NC	NS
LIF	leukemia inhibitory factor	NM_008501	6	0.0055	NC	NS
CH25H	cholesterol 25-hydroxylase	NM_009890	6	0.0232	NC	NS
PLP1	proteolipid protein (myelin) 1	NM_011123	NC	NS	6	0.0236
EGR2	early growth response 2	NM_010118	5	0.0062	NC	NS

The fold changes of retinal genes in C57BL6/J mouse eyes and TLR4−/− mouse eyes after infection relative to the uninfected, contralateral eye are shown. Levels of significance were determined using ANOVA and p < 0.05 was considered significant
NC no change, NS not significant

ISSN: 1471-2415