Skip to main content
Fig. 1 | BMC Ophthalmology

Fig. 1

From: Identification of H2O2 induced oxidative stress associated microRNAs in HLE-B3 cells and their clinical relevance to the progression of age-related nuclear cataract

Fig. 1

Establishment of H2O2 induced oxidative stress model in HLE-B3 cells. a The viability of HLE-B3 cells decreased after H2O2 treatment in both dose-dependent and time-dependent manner. b Cell apoptosis of HLE-B3 cells after H2O2 exposure for 12, 24 and 48 h was determined by flow cytometry with Annexin-V and PI staining. c Cell nucleus apoptosis of HLE-B3 cells after H2O2 exposure for 12, 24 and 48 h was determined by Hoechst 33,258 staining. Results are presented as mean ± SD by t-test. (n = 3) **P < 0.01, ***P < 0.001

Back to article page

BMC Ophthalmology

ISSN: 1471-2415

Contact us