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Fig. 3 | BMC Ophthalmology

Fig. 3

From: Phospholipase Cγ2 is critical for Ca2+ flux and cytokine production in anti-fungal innate immunity of human corneal epithelial cells

Fig. 3

PLCγ2 plays an important role for the elicitation of Ca2+ flux in HCECs. a-c HCECs were loaded with Fluo-3 and treated with DMEM in the presence of Ethanol, 5 × 106/ml A. fumigates hyphae or 5 × 106/ml A. fumigates hyphae in the presence of 1μMol/L inhibitor of PLCγ2-U73122. Confocal images of HCECs showed cytoplasmic calcium expression (green stain). d The corresponding results of fluoresecence intensity were measured by Image J software. Data are representative of more than three independent sets of experiments. Magnifications 400X. **p < 0.001

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