Fig. 4

Syk Plays a crucial role for the activation of Dectin-1-induced PLCγ2 in HCECs. a The phosphorylation of Syk was activated after A. fumigatus hyphae stimulation in HCECs. The activation status was measured by Western Blot at 5, 15, 30 and 45 min. b, c HCECs were preincubated for 30 min with Dectin-1 inhibitor Laminarin or syk inhibitor Piceatannol, followed by treatment with hyphae for 30 or 15 min. The activation of syk or PLCγ was assayed by Western Blot. Results shown are mean ± SD of three independent experiments. *p < 0.05, **p < 0.001