Fig. 3
From: Proteomic analysis of retinal pigment epithelium cells after exposure to UVA radiation
Identification of the differentially expressed proteins between the control and UVA irradiated ARPE-19 cells by 2-DE. a ARPE-19 cells irradiated with 10 J/cm2 UVA, followed by harvesting the cells and cell lysates. A total of 300 μg protein was then subjected to 2-DE and visualized by CBR staining. PDQuest image analysis software (Bio-Rad) was employed to explore differential expression through differential protein spotting. b Validation of identified selected proteins from 2-DE by western blot analysis. Some identified proteins include GRP78, CALR, PDI, NPM, Hsp27, ATP synthase, GAPDH, and PRDX1. β-actin was used as the loading control