Proteomic analysis of retinal pigment epithelium cells after exposure to UVA radiation

Chen, Jiunn-Liang; Hung, Chun-Tzu; Keller, Joseph Jordan; Lin, Hsien-Chung; Wu, Yu-Jen

doi:10.1186/s12886-019-1151-9

BMC Ophthalmology

Table 1 Proteins identification by LC/MS/MS

From: Proteomic analysis of retinal pigment epithelium cells after exposure to UVA radiation

Pot no	Protein name	Accession.no	Calculate Mr/pI	Peptide matched	Sequence covered %	MASCOT score	Regulation (fold- change)	Cellular component	Protein function
1	Nucleophosmin	P06748	32.55/4.64	16	33	103	+ 2.64	Nucleus/ Cytoplasm	Cell proliferation,/apoptosis
2	Tropomyosin alpha-4 chain	P67936	28.5/4.67	4	18	61	+ 2.31	Cytoplasm	Cytoskeleton
3	Heat-shock protein 27	P04792	22.76/5.98	13	37	245	−1.92	Cytoplasm	Stress Response
4	Tubulin beta-4 chain	P04350	49.55/4.78	2	6	151	+ 2.72	Cytoplasm	Cytoskeleton
5	Protein disulfide-isomerase	P07237	57.08/4.76	7	15	89	+ 2.17	Endoplasmic Reticulum	Stress Response
6	78 kDa glucose-regulated protein	P11021	72.28/5.07	41	45	556	+ 4.19	Endoplasmic Reticulum	Stress Response
7	Triosephosphate isomerase	P60174	26.65/6.45	3	10	120	−2.08	Cytoplasm	Glycolysis
8	Bifunctional purine biosynthesis protein PURH	P31939	64.57/6.27	1	2	44	+ 2.64	Mitochondrion	Purine biosynthesis
9	Cell division protein kinase 10	Q15131	41.01/9.06	4	3	36	−3.08	Nucleus	Cell proliferation
10	L-lactate dehydrogenase A chain	P00338	36.66/8.44	12	23	145	−1.72	Cytoplasm	Glycolysis
11	Serum albumin precursor	P02768	69.32/5.92	31	11	194	+ 1.57	Cytoplasm	colloidal osmotic pressure of blood
12	Protein disulfide-isomerase A3	P30101	56.74/5.98	8	12	84	+ 2.72	Endoplasmic Reticulum	Stress Response
13	AH receptor-interacting protein (AIP)	O00170	37.64/6.09	2	6	49	−4.58	Cytoplasm	Protein folding
14	PDZ and LIM domain protein 1	O00151	36.04/6.56	4	13	59	−1.58	Cytoplasm	Cytoskeleton
15	PDZ and LIM domain protein 1	O00151	36.04/6.56	6	17	50	−1.56	Cytoplasm	Cytoskeleton
16	Heat-shock protein 27	P04792	22.76/5.98	83	59	874	−1.79	Cytoplasm	Stress Response
17	Heat-shock protein 27	P04792	22.76/5.98	25	31	121	−1.51	Cytoplasm	Stress Response
18	Proteasome subunit alpha type-2	P25787	25.99/6.92	16	38	111	−1.67	Nucleus/ Cytoplasm	Cell cycle,/apoptosis
19	Putative GTP-binding protein 9	Q9NTK5	44.71/7.64	11	8	38	−1.51	Nucleus/ Cytoplasm	ATP catabolic process
20	Fructose-bisphosphate aldolase A	P04075	39.39/8.3	22	43	211	−1.71	Cytoplasm	Glycolysis
21	Glyceraldehyde-3-phosphate dehydrogenase	P04406	36.03/8.57	17	34	67	−1.92	Cytoplasm	Glycolysis
22	Hydroxyacyl-coenzyme A dehydrogenase	Q16836	34.25/8.88	16	19	69	−1.52	Mitochondrion	Fatty acid metabolism
23	Alpha crystallin B chain	P02511	20.14/6.76	57	47	524	−1.85	Cytoplasm	Stress Response
24	Regulator of G-protein signaling 21	Q2M5E4	17.66/6.59	2	12	45	−1.72	Cytoplasm	Signal transduction inhibitor
25	Peroxiredoxin-1	Q06830	22.32/8.27	23	46	273	−1.56	Cytoplasm	Stress Response
26	ATP synthase	P25705	59.71/9.16	13	13	65	−1.58	Mitochondrion	Energy metabolism
27	Calreticulin	P27797	48.28/4.29	133	71	3246	+ 1.69	Endoplasmic Reticulum	Stress Response
28	Peptidyl-prolyl cis-trans isomerase A	P62937	18.23/7.68	8	23	129	−1.62	Cytoplasm	Protein folding
29	Peptidyl-prolyl cis-trans isomerase A	P62937	18.23/7.68	45	60	1160	−1.72	Cytoplasm	Protein folding

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ISSN: 1471-2415

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