Fig. 4

In situ hybridization performance in lateral geniculate neurons in each group (DAB× 200). The positive expression of VIP-mRNA was found in the cytoplasm of the neurons and overlapped with the blue staining nuclei, showing a brown-yellow color. At the age of 6 weeks, a control group had more positive cells, in the (b) deprivation group, there were fewer positive cells. At 9 weeks of age, c normal control group with more positive cells, in the d VIP intervention group, positive cells increased compared with the (e) sefsol intervention group and the (f) non-intervention group, but still less than the normal control group