Fig. 4
Second harmonic generation (SHG) taken by two-photon confocal microscopy in vivo. (a, e and i) Corneas from control group mice exhibited high intensity transient signals, indicating regular arrangement of corneal collagen fibers. Corneas from FK group (b, f and j) and vehicleinj FK group mice (c, g and k) exhibited weak signals, indicating collagen degradation and disarrangement of collagen fibers caused by infection and inflammation. (d, h and i) The uMSCsinj FK group mice corneas exhibited relative high intensity transient signals indicating relative regular collagen fibril arrangements compared with the other two groups. (m) Statistical analysis of average optical intensity (AOD) of SHG between groups on 14d post-injury. Values are presented as means ± SD, n = 6, *P < 0.05. Scale bar, 50 μm