Fig. 3

Representative immunofluorescence staining for f-actin, α-SMA, fibronectin and collagen type 1 of capsular bags without IOL and following AcrySof or Clareon IOL implantation. The double staining for α-SMA and f-Actin showed a partial co-localization of both markers and correlates well with a myofibroblast-like phenotype of the cells at the posterior lens capsule. For fibronectin focal and ribbon-like signals adjacent to cells at the posterior lens capsule were detected and were even more pronounced in the IOL groups. In the no-IOL group, fibronectin was predominantly located in folds of the posterior lens capsule and was hardly detected between the folds. For collagen type 1 a diffuse background staining was seen as it was secreted to the posterior capsule. In addition, in areas of the posterior lens capsule with a higher cell density or in capsule folds, an enhanced expression of collagen type 1 was observed. (magnification: 20-fold; blue, Hoechst staining)