Fig. 2
Changes of the b-wave amplitude after application of nominally 100 nM CuCl2 for control and Cav2.3-deficient mice. a Mean representative ERG traces (n = 3 consecutive sweeps) for a single retina from Cav2.3-competent mice before, after 30 min of superfusion with 100 nM CuCl2 in modified Ames-medium as well as after 30 min of washout. b Normalized mean values for retinas (n = 6 separate retinas) from Cav2.3-competent mice before and after the superfusion with 100 nM CuCl2 as well as after 30 min of washout. The b-wave amplitude was significantly increased by 28.8 ± 8.2% (p = 0.024) in Cav2.3-competent mouse retinas. c Mean representative ERG traces (n = 3 consecutive sweeps) for a single Cav2.3-deficient retina before and after 30 min of superfusion with 100 nM CuCl2 in modified Ames-medium as well as after 30 min of washout. Note, that in comparison to the competent mice no change of the b-wave amplitude was observed (all three traces line up together). d Normalized mean values for retinas (n = 6 separate retinas) from Cav2.3-deficient mice (Knockout) before and after the superfusion with 100 nM CuCl2 as well as after 30 min of washout. Note, that in comparison to the Cav2.3(+|+) mice no significant change of the b-wave amplitude was observed. The b-wave amplitude following stimulation with 100 nM CuCl2 was 101.3 ± 1.9% (n = 6)