Table 1 Comparison of nutrient solutions used for ERG recordings from bovine (Sickel-medium) or murine isolated retina (modified AMES-medium). AMES-medium was modified by increasing the pH slightly to alkaline (pH 7.7) and by adding 0.1 mM BaCl₂

NaCl

120 mM

120 mM

KCl

2 mM

3.1 mM

CaCl₂

0.15 mM

1.15 mM

MgCl₂/MgSO₄ * 7 H₂O

0.1 mM

–

D-glucose

5 mM

6 mM

MgSO₄

–

1.24 mM

KH₂PO₄

–

0.5 mM

NaH₂PO₄

1.5 mM

–

Na₂HPO₄

13.5 mM

–

NaHCO₃

–

22.6 mM (pH 7.4)

45.3 mM (pH 7.7)

L-glutamine, amino acids, vitamins and other components

–

Sigma-Aldrich Nr. A1420

Equilibration

Pure oxygen

Carbogen gasing containing 5% (v/v) of CO₂ in O₂

pH

7.8

7.4–7.7

BaCl₂

–

0.1 mM