Fig. 4

MiR-221 regulates Nrf2 pathway through SIRT1. a: hRMEC were transfected with pcDNA3.1-SIRT1 (p-SIRT1) as indicated. The mRNA level of SIRT1 was detected by qRT-PCR. (*** indicates P < 0.001). b: hRMEC were transfected with p-SIRT1 and/or miR-221 mimics as indicated and cultured under HG condition. The mRNA level of Nrf2 was detected by qRT-PCR. (* indicates P < 0.05; ** indicates P < 0.01). c: hRMEC transfected with p-SIRT1 and/or miR-221 mimics, and treated with ML385 as indicated were cultured under HG condition. Protein level of SIRT1 and Nrf2 were detected by Western Blot assay. Full-length blots are presented in Supplementary Figure 4C. Statistical analysis was conveyed and displayed in columns. (* indicates P < 0.05; ** indicates P < 0.01)