Fig. 1

Enucleated porcine eyes served as a type 1 EVCM. a, b Fluoresbrite Carboxylate Microspheres (1.0 μm) are almost the same size as Staphylococcus bacteria. c, d Fluoresbrite carboxylate microspheres (2.5% Solids-Latex; Polysciences, Inc.) were applied to the conjunctival surface at the insertion site. Twenty-five- or 27-gauge (G) trocar cannulas (27-G in this image) were inserted through the conjunctiva and sclera, followed by the placing and opening of the infusion cannula. These procedures were carefully monitored with an intraocular fiber catheter (yellow arrow). Scale bars, 20 μm