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Fig. 2 | BMC Ophthalmology

Fig. 2

From: Increased Association of Deamidated αA-N101D with Lens membrane of transgenic αAN101D vs. wild type αA mice: potential effects on intracellular ionic imbalance and membrane disorganization

Fig. 2

Size-exclusion HPLC (using a G-4000PWXL column) and SDS-PAGE analysis of WS-HMW proteins eluted in the void volume. a HPLC-protein elution profiles at 280 nm of WS-proteins from lenses of 5-month-old CRYαAN101D - and CRYαAWT mice. The green region shows the difference in the A280 profiles of WS-proteins from the two type of lenses. b Western blot analysis of the void volume peaks (constituted by the fraction no. 6 to 9 in [A]) following HPLC separation of WS-proteins from lenses of CRYαAN101D - and CRYαAWT mice. Note that in the CRYαAWT lenses, the αA-immunoreactive bands were in fractions no. 8 and 9 whereas it were in fraction no. 7 and 8 in CRYαAN101D lenses, suggesting a higher Mr of HMW proteins in the latter. c Quantification of the Western Blot using Image J. Gel images are not cropped

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BMC Ophthalmology

ISSN: 1471-2415

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