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Fig. 3 | BMC Ophthalmology

Fig. 3

From: Increased Association of Deamidated αA-N101D with Lens membrane of transgenic αAN101D vs. wild type αA mice: potential effects on intracellular ionic imbalance and membrane disorganization

Fig. 3

SDS-PAGE analysis of WI-US- and WI-UI-protein fractions of 5-month-old lenses from CRYαAN101D and CRYαAWT mice. To normalize the protein analyses, the protein fractions were isolated under identical conditions and with identical buffer volumes. Equal volumes of protein fractions from lenses of two types of mice were used during the analysis. The four fractions numbers as 1 to 4 (containing total proteins in WI-US- and WI-UI-fractions) and four fractions numbered as a to d (containing aggregated proteins with Mr. > 30 kDa) were analyzed by mass-spectrometric methods, and the results are shown in Supplementary Tables A, B, C and D. Gel images are not cropped

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