Fig. 2
From: Various cross-linking methods inhibit the collagenase I degradation of rabbit scleral tissue
Scheme of the methodical procedure. The rabbit scleral patches were cross-linked using glutaraldehyde, paraformaldehyde, riboflavin and UV-A or blue light, respectively. The patches were incubated in 1 mg collagenase I (MMP1) for 24 h in a humid chamber at room temperature. Supernatant was taken at different time points and checked for their peptide content using Fluoraldehyde o-Phthaldialdehyde (OPA). The relative fluorescence was detected by a microplate reader (excitation 340 nm/ emission 450 nm). If the cross-linking treatment increases the scleral resistance to MMP1-degradation the relative fluorescence of the supernatant is decreased because of its lesser peptide amount compared to the untreated control