Fig. 3
From: Various cross-linking methods inhibit the collagenase I degradation of rabbit scleral tissue
Comparison of different peptide standards for the calculation of scleral peptide degradation products. Collagenase I (MMP1) degraded bovine collagen (blue curve), bovine serum albumin (black curve) and porcine gelatin (red curve) were diluted, incubated with Fluoraldehyde o-Phthaldialdehyde and checked for their fluorescence intensity. The standard curves (based on fluorescence values of each indicated peptide concentration) were compared to the fluorescence intensity of the supernatant of non-cross-linked rabbit scleras after the 24 h-degradation by MMP1 (black dot). The peptide amount per 1 ml supernatant from the non-cross-linked sclera after 24 h degradation by MMP1 was 1.58 mg ± 0.04 (horizontal SD) with a relative fluorescence intensity of 0.229 ± 0.06 (vertical SD), n = 15. The means and its corresponding standard deviation are displayed. Porcine gelatin standard curve corresponds mostly to the real protein amount of the scleral patches