Fig. 10

Analysis of serpina3n protein expression. Serpina3n expression was analyzed by immunofluorescence on retinal cryosections and retinal wholemounts 3 weeks after adoptive transfer. Naive eyes were used as control. Cryosections and wholemounts were prepared and stained for serpina3n (magenta) and CD31 (green) detection. Cell nuclei were stained with Hoechst (blue). a. Expression profile of Serpina3n at the mRNA level. b Analysis of serpina3n expression by immunofluorescence on naive retinal cryosections. CB = ciliary body, OS = ora serrata. c Analysis of serpina3n expression by immunofluorescence on naive retinal wholemounts. d Analysis of serpina3n expression by immunofluorescence on EAU retinal cryosections. e Analysis of serpina3n expression by immunofluorescence on EAU retinal wholemounts. Thin arrows point to serpina3n and CD31 co-staining in a normal vessel, thick arrows point to serpina3n and CD31 co-staining at the level of an inflamed vessel. Scale bars represent 20 μm