Fig. 11

Analysis of lcn2 protein expression. Lcn2 expression was analyzed by immunofluorescence on retinal cryosections and retinal wholemounts 3 weeks after adoptive transfer. Naive eyes were used as control. Cryosections and wholemounts were prepared and stained for lcn2 (magenta) and CD31 (green) detection. Cell nuclei were stained with Hoechst (blue). a Expression profile of lcn2 at the mRNA level. b Analysis of lcn2 expression by immunofluorescence on naive retinal cryosections. c Analysis of lcn2 expression by immunofluorescence on naive retinal wholemounts. d Analysis of lcn2 expression by immunofluorescence on EAU retinal cryosections. e Analysis of lcn2 expression by immunofluorescence on EAU retinal wholemounts. f Analysis of lcn2 expression by immunofluorescence on EAU retinal wholemounts (× 40 objective). Arrows point to possible lcn2 and CD31 co-staining. Scale bars represent 20 μm