Fig. 12

Analysis of ackr1 protein expression. Ackr1 expression was analyzed by immunofluorescence on retinal cryosections and retinal wholemounts 3 weeks after adoptive transfer, as well as by flow cytometry. Naive eyes were used as control. Cryosections and wholemounts were prepared and stained for ackr1 (red or green) and CD31 (magenta) detection. Cell nuclei were stained with Hoechst (blue). a Analysis of ackr1 expression by immunofluorescence on naive retinal cryosections. b Analysis of ackr1 expression by immunofluorescence on naive retinal wholemounts. c Analysis of ackr1 expression by immunofluorescence on EAU retinal cryosections. d Analysis of ackr1 expression by immunofluorescence on EAU retinal wholemounts. e FACS analysis of ackr1 expression by naïve retinal endothelial (CD31+ Endoglin+ CD45-) cells. f FACS analysis of ackr1 expression by EAU retinal endothelial (CD31+ Endoglin+ CD45-) cells. Scale bars represent 20 μm