Fig. 14

Analysis of Lamc3 protein expression. Lamc3 expression was analyzed by immunofluorescence on retinal cryosections and retinal wholemounts 3 weeks after adoptive transfer. Naive eyes were used as control. Cryosections and wholemounts were prepared and stained for Lamc3 (green) and endoglin (magenta) detection. Cell nuclei were stained with Hoechst (blue). a Expression profile of Lamc3 at the mRNA level. b Analysis of Lamc3 expression by immunofluorescence on naive retinal wholemounts. c Analysis of Lamc3 expression by immunofluorescence on EAU retinal wholemounts. d FACS analysis of Lamc3 expression by naïve retinal endothelial (CD31+ Endoglin+ CD45-) cells. e FACS analysis of Lamc3 expression by EAU retinal endothelial (CD31+ Endoglin+ CD45-) cells. Arrows point to possible Lamc3 and endoglin co-staining. Scale bars represent 20 μm