Fig. 3

Endothelial cell markers are enriched in purified endothelial cells compared to total retina. a. Purity of sorted endothelial cells. RNA-Seq data were analyzed for the expression of endothelial, pericyte, photoreceptor, microglia/macrophage, glial and RPE cell markers to evaluate the purity of the endothelial cell samples. Each bar illustrates the relative expression of one marker by endothelial cells compared to total retinal cells (LogFoldchange). Green bars represent samples sorted with the transgenic strategy, blue bars represent samples sorted with the WT strategy. * FDR < 0,05, ** FDR < 0,01. b. Enrichment of barrier-type endothelial cell markers in the endothelial cell samples. RNA-Seq data were analyzed for the expression of endothelial cell (EC) markers previously identified as being specifically enriched in blood-brain barrier (BBB) ECs. Each bar illustrates the relative expression of one marker by ECs compared to total retinal cells (LogFoldchange). Green bars represent samples sorted with the transgenic strategy, blue bars represent samples sorted with the WT strategy. All represented FC reach statistical significance (FDR < 0,05)