Table 1 Recent genome wide expression data sets for retinal endothelial cells
Study | Method of retinal EC isolation | Method of gene expression analysis | Species | Other cells studied | Disease/ conditions |
|---|---|---|---|---|---|
Zhao et al., 2016 [6] | Cultured human retinal ECs (HRECs) | miRNA microarray | Human | / | Exposure to normal glucose or high glucose |
Savage et al., 2015 [7] | Cultured human retinal microvascular ECs (HRMEC) | RNA-Seq | Human | / | Exposure to TNF-α in the presence or absence of the NFAT-specific inhibitor INCA-6 vs vehicle-treated control |
Wang et al., 2013 [8] | Freshly isolated mouse microvessels (CD31-based magnetic purification) | Microarray | Mouse | / | Cells from rd1, Vldlr−/− and Grhl3ct/J curly tail mice (mutants with remodelling of the retinal vasculature) vs naive C75BL/6J |
Kusuhara et al., 2012 [9] | FACS based on GFP expression in P8 Tie2GFP transgenic mice | Microarray | Mouse | GFP- retinal cells | / |
Steinle et al., 2012 [10] | Cultured human retinal ECs | Microarray | Human | / | Melphalan or carboplatin treatment vs untreated cells |
Takase et al., 2012 [11] | FACS based on GFP expression in Flk1+/GFP embryos | Microarray | Mouse | Whole WT and Flk1 KO embryos | / |
Browning et al., 2011 [12] | Retinal EC cultures from 3 human donors (CD31-based magnetic purification) | Microarray | Human | Donor-matched iris and choroidal ECs + cultured human umbilical vein ECs (HUVEC) | / |
Strasser et al., 2010 [13] | Laser capture microdissection of retinal endothelial tip cells and endothelial stalk cells 24-36h after birth | Microarray | Mouse | / | / |
Abukawa et al., 2009 [14] | Conditionally immortalized rat retinal capillary ECs (TR-iBRB2) | Microarray | Rat | / | Incubation with Müller cell conditioned medium vs control |
Smith et al., 2007 [15] | Separate primary retinal EC cultures from 6 human donors (CD31-based magnetic purification) | Microarray | Human | Donor-matched choroidal ECs | Exposure to Toxoplasma gondii tachyzoïtes or LPS vs control |
Ohtsuki et al., 2005 [16] | Conditionally immortalized rat retinal capillary ECs (TR-iBRB2 and TR-iBRB9) | mRNA differential display analysis | Rat | Brain capillary ECs (TR-BBB) | / |
Silverman et al., 2005 [17] | Separate retinal EC cultures from 4 human donors (CD31-based magnetic purification) | Microarray | Human | Donor-matched iris ECs | Exposure to LPS or TNF-α vs control |
Knight et al., 2005 [18] | Immortalized rat retinal vascular ECs (SV40 large T immortalized cell line JG2/1) | Microarray | Rat | / | Exposure to Toxoplasma gondii vs control |