Fig. 3
From: β-blocker eye drops affect ocular surface through β2 adrenoceptor of corneal limbal stem cells
Effects of β1AR and β2AR antagonists on corneal epithelial wound healing and expressions of CK3, CK14, and CK19 in the corneal limbal epithelium. A The corneal epithelium was scraped with 3.0 mm trephine and treated with saline (Ctrl), the β1AR inhibitor atenolol (Aten), and the β2AR inhibitor ICI 118, 551 (ICI), and then stained with fluorescein sodium every 12 h. B A graph of the epithelial defect area was presented as the percentage of the original wound. The expressions of CK3 (C), CK14 (C), and CK19 (C) in the corneal limbal epithelium were detected by immunofluorescence staining. Moreover, the quantification of stained sections was carried out using the Image J software (D-F). Data are representative of means ± SD. **P < 0.01, ***P < 0.001