Fig. 5

Effects of the non-selective β-blocker levobunolol, the β1AR antagonist atenolol, and the β2AR antagonist ICI 118, 551 on corneal epithelial regeneration-related signaling. The corneal epithelium was scraped including the limbal region and unceasingly received eye drops of saline (Ctrl), levobunolol (Levo), atenolol (Aten), or ICI 118, 551 (ICI) for 4 times a day. At 3 days post wounding, the expression of Ki67 (A, E), and the phosphorylation of EGFR (A, E) and ERK1/2 (A, E) in the corneal epithelium including in the regenerated and limbal regions, were detected by immunofluorescence staining. Moreover, the quantification of the stained sections was carried out using the Image J software (B-D, F-H). Data are representative of means ± SD. **P < 0.01