Fig. 5

GLI interacts with Notch1/SIRT1 pathway in HG-induced HRECs. (A) The protein level of SIRT1 in HG-induced HRECs was determined by western blotting. **P < 0.01 vs. the NG group. ^##P < 0.01 vs. the HG group. (B) The protein level of SIRT1 after transfection of si-SIRT1-1/-2/NC was determined by western blotting. **P < 0.01 vs. the si-NC group. (C) The protein levels of Notch1, Hes1, and Hey2 in HG-induced HRECs transfected with si-SIRT1-1. **P < 0.01 vs. the NG group. ^##P < 0.01 vs. the HG group. ^&&P < 0.01 vs. the HG + GLI group. HG: 30 mmol/L glucose. NG: 5 mmol/L glucose. GLI: 1 μg/ml gliquidone