Fig. 3

Pathologic findings obtained using light microscopy and transmission electron microscopy. A H&E staining image at 40× magnification showing Bowman’s layer with focal thickening (red star) and detachment (red arrow). B CD31 staining image at 200× magnification showing focal vascularization of the stroma (red star). C Transmission electron microscopy image of the stroma showing focal vascularization (red star) (original magnifications: 1400×). D Transmission electron microscopy image showing some equivocal collagen fibril disarray in the Bowman’s layer (red box) (original magnifications: 1400×). H&E, Hematoxylin and eosin. *We used light microscopy Eclipse E600 (Nikon Instruments Inc., Tokyo, Japan) with Nikon DS-Ri2 camera, and software of NIS-Elements documentation. The transmission electron microscopy JEM-1400 (JEOL Inc., Tokyo, Japan) is equipped with a CMOS camera, Silicon drift detectors, filters of NCB11, ND8, ND32, and software of RUBY 2 CCD