Table 1 Sequencing primer details
From: Detection of a novel PAX6 variant in a Chinese family with multiple ocular abnormalities
Primer Name | Sequence | Amplified fragment length (bp) | Amplification reaction conditions. |
|---|---|---|---|
SG2560_F | 5′- TACAGTAAGAAATGAAGAGAGGGCGTT − 3’ | 499 | 3 min at 95 °C for PRE-denaturation,30 s at 95 °C for denaturation,30 s at 60 °C for primer annealing, 40 s at 72 °C for primer extension, |
SG 2560_R | 5′- GGGCACGGTTGCTTGGACT − 3’ | 30 cycles,and another 5 min at 72 °C for primer extension. |