Fig. 3

The 7-mer induces JNK signaling to trigger RPMI8226 cell apoptosis. 3 × 10⁵ cells were serum starved for 24 h and then exposed to 10 ng/ml VEGF and 25 μM 7-mer for the indicated time periods. a Western blotting was performed to detect the active phosphorylated forms of JNK (p-JNK) and p38 MAPK (p-p38), shown in the upper panels. Antibodies were then stripped and the blots were re-incubated with anti-JNK and anti-p38 MAPK antibodies (lower panels), respectively, to detect the levels of total JNK and p38 MAPK. b Densitometric analyses from three independent experiments are shown. c Cell were pretreated with 10 μM SP600125 or SB203580 for 10 min and then treated as described above for 24 h and apoptosis was quantified using the annexin V-FITC apoptosis detection kit. *P < 0.0001 versus 7-mer-treated cells