Fig. 5
From: A short peptide derived from pigment epithelial-derived factor exhibits an angioinhibitory effect
The 34-mer and 7-mer display angioinhibitory properties in culture. a VEGF-induced capillary-like tubular structures were observed in matrigel with phase-contrast light microscopy. The experiment was repeated three times. Magnification is × 40. b The 7-mer induces HUVEC apoptosis in the presence of VEGF. Cell apoptosis was analyzed using the Annexin V-FITC Apoptosis Detection kit according to the in situ staining protocol after treatment for 16 h. Double staining with an annexin V-FITC and the fluorescent dye Hoechst 33342 was used to visualize the apoptotic cells (green) and nuclei (blue), respectively. A representative result of four independent experiments is shown. The percentages of apoptotic cells were quantified. aP < 0.005 versus VEGF-treated cells