Fig. 3
From: The effect of A2E on lysosome membrane permeability during blue light-induced human RPEs apoptosis
The RPEs apoptotic rate in control group, A2E group, blue light group, and A2E + blue light group detected by flow cytometer used Annexin V-FITC/PI double staining method. A In this image: Q1: The left lower quadrant was Annexin V-FITC (-) and PI (-), represented viable cells. Q2: The right lower quadrant was Annexin V-FITC ( +) and PI (-), represented viable apoptotic cells. Q3: The right upper quadrant was Annexin V-FITC ( +) and PI ( +), represented non-viable apoptotic cells. Q4: The left upper quadrant was Annexin V-FITC (-) and PI ( +), represented non-viable necrotic cells. The sum of Q2 and Q3 presented apoptotic RPEs. B The comparison of RPEs apoptotic rate in different groups detected by Annexin V-FITC/PI staining method analyzed by flow cytometry. Mean ± SD., N = 3. Statistical analysis: *P < 0.05, **P < 0.01, ***P < 0.001; one-way ANOVA and LSD-t