Fig. 4
From: The effect of A2E on lysosome membrane permeability during blue light-induced human RPEs apoptosis
The RPEs apoptotic rate in control group, A2E group, blue light group, and A2E + blue light group observed by fluorescence microscope detected by TUNEL and DAPI-stained method. A The green fluorescence represented apoptotic RPE nuclei meanwhile the blue fluorescence represented all RPE nuclei. The apoptotic RPEs were stained as green/blue double fluorescence which was considered as TUNEL stained positive cells (× 200 objective, Scale bar: 20 μm). B The comparison of RPEs apoptotic rate in different groups detected by TUNEL and DAPI-stained method observed by fluorescence microscope. RPEs apoptotic rate calculation: RPEs apoptotic rate = TUNEL stained positive cells number/total cells number × 100%. Mean ± SD., N = 3. Statistical analysis: **P < 0.01, ***P < 0.001; one-way ANOVA and LSD-t