Fig. 5

The fluorescence intensity in control group, A2E group, blue light group, and A2E + blue light group stained by AO analyzed by LCS Lite. A The image of living RPEs in different groups stained by AO and observed by LSCM (× 400 objective). In living RPEs cultured in vitro, AO binding to cytoplasmic and nucleolar RNAs emits green fluorescence and accumulates in acidic vesicles like lysosomes, emits red fluorescence. The mean red fluorescence intensity was chosen as the statistics indicator to estimate LMP change in RPEs cultured in vitro affected by A2E and blue light irradiation. B The comparison of mean fluorescence intensity (red) in different groups stained by AO analyzed by LCS Lite. Mean ± SD., N = 3. Statistical analysis: ***P < 0.001; one-way ANOVA and LSD-t