Fig. 2

Representative images of PAS staining and MUC5AC staining in acute SJS/TEN patients and healthy volunteers (Scale bars = 100 μm). a PAS staining of conjunctival impression cytology in a healthy volunteer (× 200, Nelson’s grade 0). Goblet cells (green arrow) are abundant. Nonsecretory epithelial cells (red arrow) are small and round and have large nuclei with a nuclear-cytoplasmic ratio = 1:2. b-g PAS staining of conjunctival impression cytology in patients with acute SJS/TEN (× 200, Nelson’s grade 3). Nonsecretory epithelial cells (red arrow) are slightly separated and have small and pyknotic nuclei with decreased nuclear-cytoplasmic ratio, and goblet cells (green arrow) are significantly reduced. h MUC5AC staining of conjunctival impression cytology in a healthy volunteer (× 200). MUC5AC⁺ goblet cells include a non-degranulated pattern with complete cell boundaries and intracellularly packaged mucins (yellow arrow) and a degranulated pattern with blurred cell boundaries and scattered mucin particles (white arrow). i-n MUC5AC staining of conjunctival impression cytology in patients with acute SJS/TEN (× 200). MUC5AC.⁺ goblet cells were almost all in the form of non-degranulated (yellow arrow)