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Table 1 Frequency of human cytokeratins identified in spots from 2D-PAGE in a human corneal study and mouse thymic lymphoma study.

From: Attempting to distinguish between endogenous and contaminating cytokeratins in a corneal proteomic study

CKs

Type I(acidic)/II(neutral or basic)

Previously described in human non-tumour-tissue

Previously described in ocular epithelium

Location1

Total count in 102 mouse spots - %

Very likely endogenous (observed in <5% of human samples)

   

3

II

 

Cornea19,17

B

0%

4

II

Sebaceous glands, airway epithelium19

Conjunctiva17, cornea5

B

4%

7

II

Glands19

Central basal cornea17No found20

-

0%

8

II

Glands19

Cornea21, conjunctiva17

-

0%

11

I

Epidermis19

 

-

0%

12

I

 

Cornea19,2

C

0%

13

I

Glands, airway epithelium19,2

Conjuntiva25, cornea5

B

2%

15

I

Basal keratinocytes2

Limbal basal7

L

2%

17

I

Hair follicle, nails2

Cornea17

-

0%

18

I

Glands, simple epithelium19,2

Cornea21

-

0%

19

I

Glands, airway epithelium19

Conjunctiva4, basal limbus2

L

0%

20

I

Gastrointestinal tract epithelium2

 

-

0%

23

I

Non (found in pancreatic tumours)22

 

-

0%

Uncertain endogenous (observed in >5% of human samples)

   

1

II

Epidermis19

 

B

36%

2e

II

Epidermis19

 

B

78%

5

II

Epidermis, glands, airway epithelium, hair follicles19

Conjuntiva25, cornea19, 24

B

3%

6A

II

Glands, airway epithelium, hair follicles19

 

B

27%

9

I

Palmoplantar epidermis2

 

B

99%

10

I

Epidermis19

 

B

75%

14

I

Epidermis, glands, airway epithelium, hair follicles19

Conjuntiva25, cornea17

B

18%

16

I

Epidermis19

Cornea4

B

6%

  1. 1Location: C, central fraction, L, limbal fraction, B, both limbal and central fraction