Fig. 2
Expression of dnMaml1 inhibited conjunctival goblet cell differentiation and induced inflammation. a,b Periodic acid Schiff’s (PAS) and Hematoxylin staining showed that goblet cells (arrows) became evident at P9 (a) and formed clusters at P16 (b) during postnatal eye development in mice. c,d However, OSdnMaml1 revealed hyperplasia in conjunctival epithelium and failed to form goblet cells. e,g Conjunctival goblet cells were identified by positive signals (arrows) using anti-Muc 5a/c antibody. Most Muc5ac+ goblet cells clustered around forniceal and bulbar conjunctival region in P16 (e). By contrast, the expression of dnMaml1 led to total absence of Muc5ac+ goblet cells in the same region (g). f,h In addition, very few CD45+leukocytes were detected in the naïve ocular surface (f). By contrast, the expression of dnMaml1 led to abundant CD45+leukocyte infiltration into the subconjunctival space (h). cb, ciliary body; cjs, conjunctival sac; co, cornea; el, eyelid; ir, iris; le, lens; re, retina. (Courtesy of Development)