- Research article
- Open Access
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Diabetic macular morphology changes may occur in the early stage of diabetes
BMC Ophthalmologyvolume 16, Article number: 12 (2016)
The purpose of this study was to observe whether invisible morphological changes are presented in the two types of diabetes mellitus patients without diabetic retinopathy.
Twenty-six type 1 diabetes mellitus (T1DM) patients and 34 type 2 diabetes mellitus (T2DM) patients without diabetic retinopathy (DR) were recruited for this study. They underwent complete examinations that included stereoscopic color fundus photography and optical coherence tomography (OCT). The OCT patterns were used to measure the macular retinal thickness (RT), the ganglion cell and inner plexiform layer (GC-IPL) complex thickness, the inner nuclear layer (INL) thickness, the outer nuclear layer (ONL) thickness and the subfoveal choroidal thickness (SFCT) using the enhanced depth imaging (EDI) patterns and the retinal fiber layer (RNFL) thickness around the optic disc. All results were compared to those of age- and sex-matched control groups.
In the patients with T1DM, the mean RT and GC-IPL complex thicknesses were significantly thinner than those of the control group (p < 0.05). The RNFL was found to be thinner at the 9 o’clock position around the optic disc in the patients compared with the control group. The SFCTs were similar in the controls and subjects. The INL and ONL were decreased in parts of the pericentral and peripheral areas in the T1DM patients (p < 0.05) and increased in the T2DM patients (p < 0.05).
This study demonstrated that in short-duration T1DM patients, the layers of the retina are affected and that the neural tissue has begun to be lost. As diabetes develops, neurodegeneration may cause vascular permeability, which causes thickening of the retinal layers.
The global incidence of diabetes mellitus (DM) is increasing, and the number of patients is expected to reach 3.66 billion by 2030 . Diabetic retinopathy (DR) is one of the most common and severe complications of DM and is also the leading cause of blindness. In America, 40 % of type 2 diabetes mellitus (T2DM) patients and 86 % of type 1 diabetes mellitus (T1DM) patients suffer from DR [2, 3]. In China, the incidence of DR is 27.9 % in urban areas and 43 % in rural areas [4, 5]. Thus, the early detection of DR is important for the preservation of useful acuity in later life.
The present examinations of DR included direct ophthalmoscopy, fundus photography, optical coherence tomography (OCT) and fluorescence angiography. OCT is a noninvasive technique that was developed to be rapid in terms of its hardware and software attributes, and OCT exhibits high levels of accuracy and definition in the detection of early and small changes in retinal morphology. The newly updated Carl Zeiss OCT software version 6.0 integrates FastTrac retinal tracking, FoveaFinder, ganglion cell analysis, ONH and RNFL OU analyses, enhanced depth imaging (EDI) and other powerful functions that enable the acquisition of reliable data for clinical research.
In addition to the retinal layers, the choroid has also attracted much interest due to its vascular structure and supply of oxygen and nutrients to the outer retina. The EDI pattern is also useful for clearly displaying choroid thickness and structural abnormalities . Increases and decreases in macular and choroid thicknesses have been observed in patients with and without diabetic retinopathy in previous studies, and different mechanisms have been found to be responsible for these changes [7–11]. Our study aimed to fully utilize OCT to identify the types of morphological changes in DM patients without retinopathy.
This was a cross-sectional, case–control study. The design of this study followed of the principles of the Declaration of Helsinki and was approved by the Institutional Review Board of the RuiJin Hospital. Written informed consent was obtained from all participants or their parents (children who were under 16 years old). The patients were recruited from the outpatient clinic of the department of Endocrinology Medicine of the RuiJin Hospital affiliated with the Shanghai Jiao Tong University School of Medicine (Shanghai, China) between October 2013 and November 2014. The patients were diagnosed with type 1 or type 2 diabetes mellitus by an endocrine specialist and were treated with insulin or other medicines. The normal control subjects were selected from the medical examination center of the same hospital. They were age- and sex-matched to the patients; were free of all diagnoses of ocular disease, diabetes, or other systemic disease; and underwent a complete ophthalmologic examination that included a visual acuity assessment, slit-lamp examination with a handheld lens (Super Field; Volk Optical, Inc., Mentor, OH), intraocular pressure (IOP) assessment with a noncontact tonometer, and refraction, color fundus photograph (TRC-50IX; Topcon Corp.), and Cirrus high-definition optical coherence tomography (HD-OCT, Carl Zeiss Meditec, Dublin, California). The inclusion criteria were as follows: 1) a diagnosis of T1DM or T2DM with no clinical retinopathy as observed through dilated pupils by an experienced doctor using a stereoscopic slit lamp with a handheld lens; 2) a best-corrected visual acuity ≥20/25; and 3) refractive errors below -6 or above +3 diopter equivalent spheres. The exclusion criteria were significant media opacity, glaucoma, uveitis, ocular trauma or surgery history, and other serious systemic diseases. Additionally, age, sex, duration of diabetes (in years) were recorded, and fasting glucose and HbA1c levels were collected from the patients’ recent blood reports (Tables 1 and 2).
A Cirrus HD-OCT 4000 (software Version 6.0) was used to obtain the macular images. The patients placed their chins in a chinrest with their foreheads contacting a headrest and fixated on a green target. The macular cube 512 × 128 scan protocol was used to acquire all 128 OCT B-scans that covered an area of 6 × 6 mm and were 2 mm in length centered on the fovea. A mean retinal thickness (RT) map of nine zones from the internal limiting membrane to the retinal pigment epithelium was automatically measured. We defined the foveal area (A1) as a central circle with a diameter of 1 mm, the pericentral area (A2-A5) as a donut-shaped ring centered on the fovea with an inner diameter of 1 mm and an outer diameter of 3 mm, and the peripheral area (A6-A9) as a ring with an inner diameter of 3 mm and an outer diameter of 6 mm (Fig. 1a).
The ganglion cell analysis algorithm was used to measure the GC-IPL thickness within a 14.13 mm2 elliptical annulus centered on the fovea based on the 3-dimensional data generated from the macular cube 512 × 128 scan protocol. The outer boundary of the retinal nerve fiber layer (RNFL) and the outer boundary of the inner plexiform layer were automatically segmented by the algorithm. The GC-IPL thicknesses of the superotemporal, superior, superonasal, inferotemporal, inferior and inferonasal sectors were automatically measured (Fig. 1b).
A one-line raster scan of the EDI pattern was performed to obtain high-quality images of the retina and macular choroid. The scan line was as close as possible to the fovea in the horizontal (0°) and vertical (90°) directions. Only images with signal strengths exceeding 6 were selected for the study. The thicknesses of the inner nuclear layer (INL) and the outer nuclear layer (ONL) were measured manually every 0.5 mm from the center to the periphery in the nasal and temporal directions. Each measurement was performed three times, and the mean values were determined. The choroid thickness was defined as the vertical distance from the hyper-reflective line of Bruch’s membrane to the hyper-reflective line of the inner surface of the sclera. The subfoveal choroidal thickness (SFCT) was measured in the horizontally and vertically scanned images to obtain a mean value (Fig. 2).
The optic disc cube scan protocol was used to obtain the RNFL measurements from the T1DM patients. There were 200 A-scans, and from each of 200 B-scans, the software automatically determined the center of a disc with a radius of 1.73 mm. The RNFL thicknesses of 12 sectors of the optic disc that were defined like the hours on a clock were measured and calculated using this software. The data were recorded in the superior-to-temporal, inferior-to-nasal, and clockwise directions (Fig. 1c).
Statistical analyses were performed using SPSS software version 20.0 for IOS (IBM Corporation, Chicago, IL, USA). All data are expressed as the mean ± standard deviation (SD). A chi square test was used to assess the difference in the female/male ratio between the patients and controls. Analysis of variance (ANOVA) was used to assess the differences in mean age, BCVA, IOP, fasting glucose level, HbA1c and DM duration between the patients and controls. The mean RT, GC-IPL complex, INL, ONL and RNFL thicknesses in the diabetic patients and controls were compared using independent t tests. The results were considered statistically significant when p < 0.05.
In total, 26 T1DM patients and 34 T2DM patients were analyzed in this study. Age- and sex-matched controls were 25 and 36 respectively (Table 1). Both eyes of all participants were scanned, and the left eyes were selected for analysis. The mean RTs of all nine of the zones of the T1DM subjects were thinner than those of the control group, and the differences in zones A2 to A9 were statistically meaningful (p < 0.05); however, no significant changes were found in the T2DM patients (Table 3). All six sectors of the GCIPL complex in the T1DM patients were thinner than those in the control group, but these values were similar in the T2DM patients and controls (Table 4). The INL and ONL of the T1DM patients were both thinner at some of the tested points in the periphery and pericentral areas (p < 0.05) and slightly thicker in the fovea; the latter difference was not statistically meaningful (Figs. 3 and 4). In contrast, the opposite pattern of results was observed in the T2DM patients who displayed thicker layers (Figs. 5 and 6).
The SFCTs were 294.44 ± 60.96 μm in the T1DM patients and 303.16 ± 60.23 μm in the T1DM control group (p = 0.64). In the T2DM patients and their control group, these values were 284 ± 84.89 μm and 254 ± 67.36 μm, respectively (p = 0.32).
The RNFL thicknesses of the optic discs of the T1DM patients were significantly decreased at the 9 o’clock position (p = 0.005).
Previous studies have reported changes in the macular retinal thickness and the thicknesses of different layers in patients with and without diabetic retinopathy [7–11]. The early diagnosis and early detection of functional changes related to DR that occur prior to retinal morphology changes are important for preventing DR. Some researchers have found that retinal neurodegeneration may occur in DR before any microcirculatory abnormalities can be detected [12, 13]. Neural apoptosis and the loss of ganglion cell bodies and glial reactivity are now considered to be the main factors in DR [14, 15].
In this study, we observed significant decreases in the RTs of the A2 to A9 zones and the thickness of all sectors of the GC-IPL complex in the macular zones of the T1DM patients. Additionally, among the tested points, N2.0 in the INL and N0.5, N2.0, T0.5, T1.0, and T2.0 in the ONL were found to be decreased in the patients. In the RNFL, the 9 o’clock position, i.e., the nasal section, was found to be reduced in the patients. In sharp contrast to the T1DM group, we did not observe many positive findings in the T2DM patients. No measured RT or GC-IPL complex thicknesses were found to be different between the T2DM patients and controls. However, increased thicknesses in the testing points N1.5, N2.0, T1.0, T1.5, and T2.0 in the INL and N0.5 and N1.0 in the ONL were observed. In the SFCT, we did not observe any changes in either the T1DM or T2DM patients.
To our knowledge, there were no previous ophthalmological studies of newly diagnosed T1DM patients. In the present study, the mean disease durations were 2.1 ± 3.0 years for the type 1 DM patients and 10.17 ± 6.84 years for the type 2 DM patients (p < 0.05). The T2DM patients exhibited no significant differences in the measures of retinal thickness or the GC-ILP complex, but these measures were decreased in the T1DM patients. Similar results have been reported in other studies [8, 9], which have detected either thickening or thinning of the retina. The majority of these reports found that the retina becomes thinner when DR is present [9, 16]. Together, all of the above-mentioned findings lead to the conclusion that the retinal and ganglion cell layer thicknesses are correlated with DM duration. In the initial few years of diabetes, the macular thickness decreases due to neural tissue loss, but as diabetes progresses, these thicknesses gradually increase due to vascular permeability in the retina [10, 11]. In patients without DR, Bronson-Castain et al.  found that the RT was decreased in a group of adolescent T2DM patients with a mean disease duration of 2.1 years. However, the authors of this study expressed doubt about the neural tissue theory based on the short duration of DM in their adolescent patients.
Our results indicated that the neurodegenerative effects on the retina probably occurred prior to vasculopathy in the early stages of diabetes based on the variations in the ages and disease durations of our population. Therefore, our results do not mean that there were no changes in the RTs of the T2DM patients; rather, the thickening of other layers, such as the INL and ONL, which were observed in the present study, probably masked these changes.
Due to technical limitations, we were unable to directly measure the ganglion cell layer thickness, but the newly updated OCT software provides accurate data about the GC-IPL complex for clinical studies.
Other studies have also observed ganglion cell loss in DR and nonproliferative diabetic retinopathy (NDR) patients , and axonal loss may lead to the thinning of the RNFL . Moreover, both ganglion cells loss and axonal loss are correlated with HbAc1 and duration  and worsen as disease severity increases. The pericentral macular area has been found to be significantly thinner in patients with minimal DR [18, 20] and thicker in patients with preproliferative diabetic retinopathy , which suggests that neuronal abnormalities may precede vascular abnormalities .
We found that the RNFL around the optic disc was thinner in the patients than in the controls only at the 9 o’clock position, which may prove that RNFL degeneration occurs in the onset stage of diabetes [14, 21]; however, it is difficult to determine which area is the first to be affected.
In the inner nuclear layer, the pericentral and peripheral macular thicknesses were decreased in the T1DM patients compared to the controls, whereas they were thicker in the T2DM group, and the same pattern of alterations was observed in the outer nuclear layer. These results confirm our speculation that neurodegeneration occurs during the early stages of disease and that the vascular permeability increases with disease duration and leads to increased retinal thickness. However, the differences were not significantly meaningful at all of the tested points; thus, we may need to increase our sample size. Only manual measurements and horizontal EDI scans were used to determine the INL and ONL thicknesses, which limited our statistical power. Continuous follow-up of these subjects is also needed for further study.
The reports regarding choroidal thickness (CT) in diabetes patients seem to be consistent in that they have all reported decreased CTs in subjects with proliferative diabetic retinopathy or diabetic macular edema and a lack of significant changes in NDR patients [22, 23] and T1DM children without DR . The breakdown of the blood-retinal barrier, loss of retinal vasculature integrity and the presence of hemodynamic abnormalities may contribute to changes in the choroidal thicknesses of diabetic eyes [25, 26]. In our study, the SFCTs of the T1DM and T2DM patients were similar to those of the controls, which may suggest that the choroid tissue is affected later than the neuroretinal layer in diabetic retinopathy.
We detected morphological changes in DM patients with Cirrus HD-OCT, and the remarkable results observed in the T1DM patients confirmed that the loss of neural tissue begins in the early stages of diabetes. As diabetes develops, neurodegeneration may be masked by changes in vascular permeability that cause thickening of the retinal layers.
best-corrected visual acuity
enhanced depth imaging
ganglion cell and inner plexiform layer
inner nuclear layer
nonproliferative diabetic retinopathy
optic coherence tomography
outer nuclear layer
retinal fiber layer
subfoveal choroid thickness
type 1 diabetes mellitus
type 2 diabetes mellitus
Wild S, Roglic G, Green A, Sicree R, King H. Global prevalence of diabetes: estimates for the year 2000 and projections for 2030. Diabetes Care. 2004;27(5):1047–53.
Kempen JH, O’Colmain BJ, Leske MC, Haffner SM, Klein R, Moss SE, et al. The prevalence of diabetic retinopathy among adults in the United States. Arch Ophthalmol. 2004;122(4):552–63. Chicago, Ill : 1960.
Roy MS, Klein R, O’Colmain BJ, Klein BE, Moss SE, Kempen JH. The prevalence of diabetic retinopathy among adult type 1 diabetic persons in the United States. Arch Ophthalmol. 2004;122(4):546–51. Chicago, Ill : 1960.
Xie XW, Xu L, Wang YX, Jonas JB. Prevalence and associated factors of diabetic retinopathy. The Beijing Eye Study 2006. Graefes Arch Clin Exp Ophthalmol. 2006;246(11):1519–26.
Wang FH, Liang YB, Zhang F, Wang JJ, Wei WB, Tao QS, et al. Prevalence of diabetic retinopathy in rural China: the Handan eye study. Ophthalmol. 2009;116(3):461–7.
Querques G, Lattanzio R, Querques L, Del Turco C, Forte R, Pierro L, et al. Enhanced depth imaging optical coherence tomography in type 2 diabetes. Invest Ophthalmol Vis Sci. 2012;53(10):6017–24.
Pires I, Santos AR, Nunes S, Lobo C. Macular thickness measured by stratus optical coherence tomography in patients with diabetes type 2 and mild nonproliferative retinopathy without clinical evidence of macular edema. Ophthalmologica. 2013;229(4):181–6.
Demir M, Oba E, Dirim B, Ozdal E, Can E. Central macular thickness in patients with type 2 diabetes mellitus without clinical retinopathy. BMC Ophthalmol. 2013;13:11.
van Dijk HW, Kok PH, Garvin M, Sonka M, Devries JH, Michels RP, et al. Selective loss of inner retinal layer thickness in type 1 diabetic patients with minimal diabetic retinopathy. Invest Ophthalmol Vis Sci. 2009;50(7):3404–9.
Araszkiewicz A, Zozulinska-Ziolkiewicz D, Meller M, Bernardczyk-Meller J, Pilacinski S, Rogowicz-Frontczak A, et al. Neurodegeneration of the retina in type 1 diabetic patients. Pol Arch Med Wewn. 2012;122(10):464–70.
Cabrera DeBuc D, Somfai GM. Early detection of retinal thickness changes in diabetes using Optical Coherence Tomography. Med Sci Monit. 2010;16(3):Mt15–21.
Villarroel M, Ciudin A, Hernandez C, Simo R. Neurodegeneration: An early event of diabetic retinopathy. World J Diabetes. 2010;1(2):57–64.
Antonetti DA, Klein R, Gardner TW. Diabetic retinopathy. N Engl J Med. 2012;366(13):1227–39.
Lecleire-Collet A, Tessier LH, Massin P, Forster V, Brasseur G, Sahel JA, et al. Advanced glycation end products can induce glial reaction and neuronal degeneration in retinal explants. Br J Ophthalmol. 2005;89(12):1631–3.
Barber AJ. A new view of diabetic retinopathy: a neurodegenerative disease of the eye. Prog Neuropsychopharmacol Biol Psychiatry. 2003;27(2):283–90.
Biallosterski C, van Velthoven ME, Michels RP, Schlingemann RO, DeVries JH, Verbraak FD. Decreased optical coherence tomography-measured pericentral retinal thickness in patients with diabetes mellitus type 1 with minimal diabetic retinopathy. Br J Ophthalmol. 2007;91(9):1135–8.
Bronson-Castain KW, Bearse Jr MA, Neuville J, Jonasdottir S, King-Hooper B, Barez S, et al. Adolescents with Type 2 diabetes: early indications of focal retinal neuropathy, retinal thinning, and venular dilation. Retina. 2009;29(5):618–26. Philadelphia, Pa.
van Dijk HW, Verbraak FD, Kok PH, Garvin MK, Sonka M, Lee K, et al. Decreased retinal ganglion cell layer thickness in patients with type 1 diabetes. Invest Ophthalmol Vis Sci. 2010;51(7):3660–5.
Oshitari T, Hanawa K, Adachi-Usami E. Changes of macular and RNFL thicknesses measured by Stratus OCT in patients with early stage diabetes. Eye (Lond). 2009;23(4):884–9.
Park HY, Kim IT, Park CK. Early diabetic changes in the nerve fibre layer at the macula detected by spectral domain optical coherence tomography. Br J Ophthalmol. 2011;95(9):1223–8.
Martin PM, Roon P, Van Ells TK, Ganapathy V, Smith SB. Death of retinal neurons in streptozotocin-induced diabetic mice. Invest Ophthalmol Vis Sci. 2004;45(9):3330–6.
Regatieri CV, Branchini L, Carmody J, Fujimoto JG, Duker JS. Choroidal thickness in patients with diabetic retinopathy analyzed by spectral-domain optical coherence tomography. Retina. 2012;32(3):563–8. Philadelphia, Pa.
Unsal E, Eltutar K, Zirtiloglu S, Dincer N, Ozdogan Erkul S, Gungel H. Choroidal thickness in patients with diabetic retinopathy. Clin Ophthalmol. 2014;8:637–42. Auckland, NZ.
Sayin N, Kara N, Pirhan D, Vural A, Ersan HB, Onal H, et al. Evaluation of subfoveal choroidal thickness in children with type 1 diabetes mellitus: an EDI-OCT study. Semin Ophthalmol. 2014;29(1):27–31.
Cunha-Vaz J, de Abreu JR F, Campos AJ. Early breakdown of the blood-retinal barrier in diabetes. Br J Ophthalmol. 1975;59(11):649–56.
Ciulla TA, Harris A, Latkany P, Piper HC, Arend O, Garzozi H, et al. Ocular perfusion abnormalities in diabetes. Acta Ophthalmol Scand. 2002;80(5):468–77.
This work was supported by the National Nature Science Foundation of China grants 81170860 and 81470639 and the Shanghai Nature Science Foundation grant 14411968400.
The authors declare that they have no financial competing interests.
All authors conceived of and designed the experimental protocol. XS and YY contributed to the study design and performed critical revision of the manuscript for important intellectual content. YC and JL performed the eye examinations and analyzed the data. YC wrote the paper. All authors have read and approved the final manuscript.