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Increased METTL3 expression and m6A RNA methylation may contribute to the development of dry eye in primary Sjögren’s syndrome

Abstract

Background

Primary Sjögren’s syndrome (pSS) is a chronic autoimmune disorder defined by xerostomia and keratoconjunctivitis sicca, and its etiology remains unknown. N6-methyladenosine (m6A) is the predominant posttranscriptional modification in eukaryotic mRNAs and is dynamically regulated by m6A regulators. Dysregulation of m6A modification is closely associated with several autoimmune disorders, but the role of m6A modification in pSS remains unknown. This study investigated the potential role of m6A and m6A-related regulators in pSS patients with dry eye.

Methods

This cross-sectional study included forty-eight pSS patients with dry eye and forty healthy controls (HCs). Peripheral blood mononuclear cells (PBMCs) were isolated, and the level of m6A in total RNA was measured. The expression of m6A regulators was determined utilizing real-time PCR and western blotting. The serological indicators detected included autoantibodies, immunoglobulins (Igs), complement factors (Cs), and inflammatory indicators. Dry eye symptoms and signs were measured, including the ocular surface disease index, Schirmer’s test (ST), corneal fluorescein staining score (CFS), and tear break-up time. Spearman’s correlation coefficient was employed to assess the associations of m6A and m6A-related regulator expression with clinical characteristics.

Results

The expression level of m6A was markedly increased in the PBMCs of pSS patients with dry eye compared to HCs (P value<0.001). The relative mRNA and protein expression levels of the m6A regulators methyltransferase-like 3 (METTL3) and YT521-B homology domains 1 were markedly elevated in pSS patients with dry eye (both P value<0.01). The m6A RNA level was found to be positively related to METTL3 expression in pSS patients (r = 0.793, P value<0.001). Both the m6A RNA level and METTL3 mRNA expression correlated with the anti-SSB antibody, IgG, ST, and CFS (all P values < 0.05). The m6A RNA level was associated with C4 (r = -0.432, P value = 0.002), while METTL3 mRNA expression was associated with C3 (r = -0.313, P value = 0.030).

Conclusions

Our work revealed that the upregulation of m6A and METTL3 was associated with the performance of serological indicators and dry eye signs in pSS patients with dry eye. METTL3 may contribute to the pathogenesis of dry eye related to pSS.

Peer Review reports

Introduction

Primary Sjögren’s syndrome (pSS) is a clinically common autoimmune disorder characterized by lymphocyte infiltration of exocrine glands (primarily the lacrimal and salivary glands) [1]. Glandular inflammation and tissue impairment eventually give rise to disturbances of secretory and clinical manifestations of dryness, including dry eye and xerostomia [2,3,4]. The pathogenesis of pSS is still poorly understood, and genetic and epigenetic factors have been considered to affect pSS development [5]. Existing research found irregular DNA methylation in pSS patients, and a genome-wide DNA methylation study recognized 1977 hypomethylated and 842 hypermethylated differentially methylated positions in pSS patients [6]. In addition to aberrant DNA methylation, increased miR-146a expression was also observed in peripheral blood mononuclear cells (PBMCs) from pSS patients [7]. A study showing histone hypoacetylation in pSS patients supports the concept that epigenetic factors contribute to the disease’s pathogenesis [8]. These studies show that epigenetic modifications promote the pathogenesis of pSS, but whether N6-methyladenosine (m6A) methylation is involved in epigenetic regulation in pSS with dry eye pathogenesis remains unknown.

m6A, the methylation modification at the sixth position of adenine bases in RNA, is the most common and evolutionarily conserved mRNA modification in eukaryotes [9]. m6A can affect RNA metabolism from some aspects, including mRNA splicing, mRNA stability and translation efficiency. The m6A modification is dynamically regulated by three groups of enzymes called methyltransferases (writer), demethylases (eraser) and binding proteins (reader) [10]. Methyltransferase-like 3 (METTL3), as the main RNA methyltransferase, forms a methyltransferase complex with its auxiliary partners methyltransferase-like 14 (METTL14) and Wilms tumor 1-associated protein (WTAP) to catalyze m6A modification [11]. It is well known that fat mass and obesity-associated protein (FTO) and alkylation repair homolog protein 5 (ALKBH5) are involved in removing m6A methylation [12]. In addition, m6A methylation is recognized via readers, including YTH and IGF2BP family proteins and affect the degradation and translation of downstream RNA [13, 14]. Recently, emerging studies have indicated that m6A modification is connected to some vital biological processes, especially inflammatory and autoimmune responses [15,16,17]. METTL3-mediated mRNA m6A methylation promotes dendritic cell activation [18]; B-cell-specific absence of METTL14 results in the B cell development defect [19]; WTAP promotes the differentiation of thymocytes [20]; FTO silencing inhibits macrophage polarization [21]. Moreover, ALKBH5 deficiency alleviates CD4 + T cell responses [22]; the deletion of YTHDF1 promotes the cross presentation of tumour antigens [23]. These results indicated that m6A may play a complicated role in pSS.

The objective of our research aimed to investigate the potential role of m6A modification in pSS with dry eye.

Materials and methods

Patients and controls

This cross-sectional study enrolled 48 pSS patients with dry eye from the First Affiliated Hospital of Chongqing Medical University from February 1, 2021, to April 1, 2022. These pSS patients accompanying with dry eye were diagnosed with the 2002 US-EU Consensus Group Criteria and the 2017 Dry Eye Workshop II Diagnostic Methodology Report strictly [24, 25]. The pSS patients were diagnosed by an ophthalmologist and a rheumatologist. The patients diagnosed as any other autoimmune diseases including rheumatoid arthritis (RA) and systemic lupus erythematosus, severe infection or taking immunomodulatory therapy previously were excluded. As the control group, forty healthy volunteers matched by gender and age were chosen. Approval for the present study was obtained from the Ethics Committee of the First Affiliated Hospital of Chongqing Medical University (2020 − 765).

Measurement of serological indicators

Serum samples were routinely tested by the clinical pathology laboratory, including antinuclear antibodies (ANA), anti-SSA autoantibody, anti-SSB autoantibody, rheumatoid factor (RF), immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM), complement 3 (C3), complement 4 (C4), C-reaction protein (CRP), and erythrocyte sedimentation rate (ESR).

Ophthalmological evaluation

The dry eye symptoms and signs of pSS patients were examined via ocular surface disease index (OSDI) questionnaires. Patients with a score over 13 were diagnosed with symptomatic dry eye. Tear break-up time (TBUT), corneal fluorescein staining score (CFS), and Schirmer’s test (ST) were determined to examine the tear film. The TBUT and CFS evaluation was conducted in a room with low lighting. Using a fluorescein strip (Liaoning Meizilin Pharmaceutical Co. Ltd., Tianjin, China), fluorescein was applied to the lower conjunctival sac. The subjects were asked to blink, and the time before the first fault shown in the stained tear film was recorded. Under cobalt blue light, the CFS was measured utilizing the Oxford scale [26]. ST was carried out without topical anesthesia to assess the tear production of each individual. Filter paper (Liaoning Meizilin Pharmaceutical Co. Ltd., Tianjin, China) was applied for five mins. Readings were expressed as wetting millimeters.

Extraction of lymphocytes

Peripheral blood samples (5 mL) were obtained, and then PBMCs were isolated by using lymphocyte separation medium (LDS1075, TBD, China) within two hours. The isolated PBMCs were divided into several small tubes and then separately preserved in TRIzol reagent (Roche, Switzerland) and RIPA buffer (P0013B, Beyotime, China). The isolated PBMCs samples were stored in liquid nitrogen until use.

RNA isolation and real-time qPCR

Total RNA was isolated from the PBMCs of all participants with TRIzol reagent based on the manufacturer’s protocol. A spectrophotometer (NP80-Touch, Agilent Technologies) was employed to assess the purity and concentration of total RNA. Samples containing 1.0 µg of RNA were purified and synthesized into cDNA using RT Master Mix for qPCR (HY-K0511, MCE, USA). The cDNA was amplified employing SYBR Green qPCR Master Mix (HY-K0522, MCE, USA), and the fluorescent signal was monitored by an Applied Biosystems 7500 System. All primer sequences utilized in this work are displayed in Table 1. The relative expression of m6A methylation-related genes was normalized to the internal reference and assessed through the 2−ΔΔCT method.

Table 1 Primers used in this study

Western blot analysis

The preserved protein samples from the PBMCs were isolated with RIPA lysis buffer (P0013B, Beyotime, China) containing a protease inhibitor cocktail (ST507, Beyotime, China), and the content was quantified by the BCA assay (P0012S, Beyotime). Lysates with 10 µg of total protein were isolated on 4–20% SDS‒PAGE gels and subsequently placed onto polyvinylidene fluoride membranes, according to the conventional method. After blocking with fat-free milk for one hour, the membranes were incubated with primary antibody overnight at room temperature. Primary antibodies against GAPDH (ab181602, Abcam, USA), METTL3 (ab195352, Abcam, USA), and YTHDF1 (ab220162, Abcam, USA) were used. Then, the membranes were rinsed with washing buffer three times and incubated for one hour with the secondary antibody (ab97051, Abcam, USA). The protein blots were visualized with an enhanced chemiluminescence kit (P0018FS, Beyotime, China). Protein expression was semi quantitatively analyzed with ImageJ software.

Quantification of RNA m6A

The m6A RNA methylation level was determined by utilizing the EpiQuik m6A RNA Methylation Quantification Kit (Colorimetric, Epigentek, USA) following the manufacturer’s protocols. The relative abundance of m6A was measured and calculated by the absorbance detected by a microplate spectrophotometer (Varioskan Lux, Thermo) at 450 nm.

Statistical analysis

GraphPad Prism 8.0 (GraphPad Software) was used for all statistical analyses. Numerical data with a normal distribution are indicated as the mean ± standard deviation (SD), and differences between the two groups were studied by a two-tailed Student’s t test. Numerical data with skewed distributions are indicated as the median (25th percentile-75th percentile), and differences between the two groups were examined by the Mann–Whitney U test. Categorical data are indicated as percentages and frequencies. The correlation was evaluated by Spearman’s correlation coefficient. A P value < 0.05 was regarded as statistically significant.

Results

Characteristics of pSS patients and controls

This study included 48 pSS patients and 40 HCs matched for age and gender. The mean age of 48 pSS patients was 45.67 ± 12.43 during our study. Demographic, laboratory, and clinical characteristics of pSS patients and HCs are shown in Table 2. Anti-SSA antibodies were positive in 35 of 48 (72.92%) patients, and anti-SSB antibodies were positive in 21 of 48 (43.75%) patients. There were significant differences in RF, IgA, IgG, C3, C4, and ESR levels between pSS patients and HCs (P value<0.001; P value<0.001; P value<0.001; P value<0.001; P value=0.025; P value<0.001; respectively), but no significant difference was found in IgM and CRP levels between them (P value=0.919; P value=0.187; respectively).

Table 2 Clinical characteristics data

Increased m6A levels and aberrant m6A regulators in pSS patients with dry eye

To preliminarily investigate the role of m6A levels in pSS with dry eye, we first analyzed the m6A RNA level in the PBMCs of patients with this condition. The m6A expression level in pSS patients was increased compared with that in HCs (P value<0.001) (Fig. 1a). We then determined the mRNA and protein expression levels of several enzymes participating in m6A modification. The mRNA levels of METTL3 and YTHDF1 were markedly increased in pSS patients compared with HCs, while there was no significant difference in METTL14, WTAP, ALKBH5, FTO, or YTHDF2 between the two groups (P value=0.596; P value=0.819; P value=0.078; P value=0.144; P value=0.596; respectively) (Fig. 1b-h). The protein expression levels of METTL3 and YTHDF1 in pSS patients were increased compared to those in HCs (P value=0.001, P value=0.006, respectively) (Fig. 2a-b), which is consistent with the mRNA expression results.

Fig. 1
figure 1

The increased m6 A RNA levels and elevated METTL3 and YTHDF1 mRNA levels in the PBMCs of pSS patients with dry eye. (a-h) The RNA levels of m6A/ METTL3/ METTL14/ WTAP/ ALKBH5/FTO/ YTHDF1/ YTHDF2 in PBMCs from pSS patients with dry eye and HCs. (Data are means ± SD for 48/pSS and 40/HCs; P value by Mann–Whitney U test (a-f, h) and unpaired Student’s t-test (g))

Fig. 2
figure 2

The elevated METTL3 and YTHDF1 protein levels in the PBMCs of the pSS patients with dry eye. (a) The protein level of METTL3 in PBMCs from pSS patients with dry eye and HCs. Upper panel, representative western blotting images of METTL3. Lower panel, quantification of the relative expression of METTL3. (b) The protein level of YTHDF1 in PBMCs from pSS patients with dry eye and HCs. Upper panel, representative western blotting images of YTHDF1. Lower panel, quantification of the relative expression of YTHDF1. (Data are means ± SD for 12 samples; P value by unpaired Student’s t-test (a-b))

The correlation of m6A levels with METTL3 mRNA expression in pSS patients with dry eye

We hypothesized that the aberrant m6A expression level in pSS patients with dry eye was caused by the dysregulation of m6A regulators. Thus, we analyzed the correlation between the m6A expression level and related m6A regulator expression. The m6A expression level in pSS patients with dry eye was positively associated with increased METTL3 expression (r = 0.793, P value<0.001) (Fig. 3a). There was no association between m6A and YTHDF1 (r = 0.205, P value=0.162) (Fig. 3b). Moreover, no association was found for m6A and METTL3 in HCs (r = -0.116, P value=0.467) (Supplemental Fig. 1a).

Fig. 3
figure 3

The positive correlation of m6 A RNA levels with the METTL3 mRNA level in the PBMCs of pSS patients with dry eye. (a) The correlation analyses between m6A and METTL3 mRNA levels in PBMCs from pSS patients with dry eye. (b) The correlation analysis among m6A and YTHDF1 mRNA levels in PBMCs from pSS patients with dry eye

Clinical significance of increased m6A levels and METTL3 expression in pSS patients with dry eye

To identify the clinical significance of increased m6A levels in pSS patients with dry eye, we analyzed the relationship between the m6A level and the performance of serological indicators in these patients, including ANA, anti-SSA autoantibody, anti-SSB autoantibody, RF, IgA, IgG, IgM, C3, C4, CRP, and ESR expression levels. We found that the m6A level was remarkably higher in anti-SSB-positive patients than in anti-SSB-negative patients (P value=0.009) but was not statistically correlated with ANA and anti-SSA autoantibodies (P value=0.337; P value=0.182; respectively) (Fig. 4a-c). In addition, the m6A level was positively associated with the IgG expression level (r = 0.343, P value= 0.017) and negatively associated with the C4 expression level (r = -0.432, P value = 0.002), but there was no correlation with the levels of RF, IgA, IgM, C3, CRP, and ESR (r = 0.232, P value = 0.112; r = 0.241, P value = 0.099; r = -0.026, P value = 0.861; r = -0.147, P value = 0.318; r = -0.221, P value = 0.132; r = 0.193, P value = 0.189; respectively) (Fig. 4d-k).

Fig. 4
figure 4

The correlation analyses of m6 A RNA levels in PBMCs with the serological indicators of pSS patients with dry eye. (a-c) The RNA level of m6A in PBMCs from ANA/anti-SSA/anti-SSB-positive patients with dry eye and respective-negative patients. (d-k) The correlation analysis between m6A and RF/IgA/IgG/IgM/C3/C4/CRP/ESR levels in pSS patients with dry eye, respectively. (Data are means ± SD for 48 samples; P value by Mann–Whitney U test (a-b) and unpaired Student’s t-test (c))

To further study the potential role of aberrant m6A levels in ocular surface damage, we analyzed the relationships between m6A levels and dry eye symptoms and signs in pSS patients. As shown in Table 3, a positive correlation between the m6A level and CFS was observed (r = 0.389, P value = 0.006). The m6A level was found to be negatively associated with the ST value (r = -0.364, P value = 0.011), whereas no association was found for OSDI and FBUT (r = -0.008, P value = 0.959; r = -0.203, P value = 0.167; respectively).

Table 3 Correlation analyses of m6A and METTL3 levels with dry eye signs and symptoms in pSS patients

We determined the relationships between the expression levels of METTL3 and clinical characteristics. As shown in Fig. 5; Table 3, METTL3 expression was significantly enhanced in anti-SSB-positive patients (P value= 0.012). A positive correlation of METTL3 expression with IgG (r = 0.385, P value = 0.006) and CFS (r = 0.456, P value= 0.001) and a negative correlation of METTL3 expression with C3 (r = -0.313, P value= 0.030) and ST values (r = -0.358, P value= 0.013) were found. There was no correlation between METTL3 expression and RF, IgA, IgM, C4, CRP, ESR, OSDI, or FBUT in pSS patients. (r = 0.221, P value = 0.130; r = 0.247, P value = 0.091; r = 0.122, P value = 0.408; r = -0.227, P value = 0.121; r = 0.210, P value = 0.152; r = 0.017, P value = 0.910; r = -0.066, P value = 0.658; r = 0.249, P value = 0.087, respectively).

Fig. 5
figure 5

The correlation analyses of METTL3 mRNA expression in PBMCs with the serological indicators of pSS patients with dry eye. (a-c) The RNA level of METTL3 in PBMCs from ANA/anti-SSA/anti-SSB-positive patients with dry eye and respective-negative patients. (d-k) The correlation analysis between METTL3 and RF/IgA/IgG/IgM/C3/C4/CRP/ESR levels in pSS patients with dry eye, respectively. (Data are means ± SD for 48 samples; P value by Mann–Whitney U test (a-c))

Discussion

pSS is a chronic inflammatory autoimmune disease and is characterized by exocrine gland impairment, such as in the salivary and lacrimal glands, which could result in dry mouth and eye [1]. m6A, as the most abundant modification in mRNA, is receiving increasing attention and has been found to function in viral infections and some autoimmune diseases in recent years [27, 28]. The potential role of m6A modification in pSS patients with dry eye remains largely unknown. Therefore, our work aimed to examine the levels of m6A modification and m6A-related regulator expression in the lymphocytes of pSS patients with dry eye and analyze their correlation with clinical characteristics. Our findings revealed that the expression level of the m6A modification and the mRNA and protein expression of METTL3 and YTHDF1 were all increased in pSS patients with dry eye. Moreover, the m6A level was positively correlated with METTL3 in pSS patients with dry eye. The correlation analyses indicated that m6A and METTL3 expression were correlated with anti-SSB antibody, IgG, complement, CFS, and ST. These results suggest a complicated role of m6A modifications in pSS with dry eye.

An increasing number of experiments have stated that the dysregulation of global m6A abundance and the aberrant expression of m6A regulators might be associated with various autoimmune disorders. Wang et al. [29] found that METTL3 was elevated in PBMCs from RA patients. In vitro experiments showed that METTL3 upregulation in macrophages increased the overall m6A content and that METTL3-related m6A modification was correlated with the secretion of inflammatory factors. Song et al. [30] revealed that METTL3 mutations might be a pivotal susceptibility factor for autoimmune thyroid disease. These findings indicated that aberrant m6A modification is a new regulatory mechanism in autoimmune disease. Recently, Cheng and her colleagues reported the downregulated expression of RNA-binding motif protein X-linked, ALKBH5, YTH domain-containing protein 2, and YTHDF1 in the peripheral blood samples of pSS patients [31]. The reasons for this conflicting finding might be the distinctions in the disease severity of selected patients and the investigated cell type.

The US-EU Consensus Group has suggested that one of the criteria for the diagnosis of pSS is the occurrence of anti-SSB or anti-SSA autoantibodies [24]. Previous results indicated that the anti-SSB antibody displays relatively better specificity for diagnosis [1]. The association of the expression of m6A and METTL3 with anti-SSB antibodies (not anti‐SSA antibodies) might be due to the better specificity of anti-SSB antibodies. These findings further revealed that m6A modification might contribute to the pathogenesis of pSS with dry eye. Excessive immunological and inflammatory responses are crucial features of pSS, which can be evaluated by the expression levels of IgG and complement C3 and C4 [32, 33]. Wang et al. [29] revealed that increased levels of METTL3 correlated with CRP and ESR in rheumatoid arthritis, which is similar to our findings. Our results indicated that the elevated expression of m6A and METTL3 correlated with serological immune indicators in pSS patients with dry eye. Huang et al. [19] reported that m6A-deficient mice exhibited the impairment of B cells activation and autoantibodies secretion, we speculate that aberrant m6A modification may lead to the excessive secretion of anti-SSB antibodies and IgG in B cells and the release of complements in pSS. m6A and METTL3 levels might be used as potential laboratory parameters to evaluate the systemic immune condition of pSS patients in clinical practice. Moreover, m6A methylation could be a potential candidate for the epigenetic-based treatment of pSS.

Previous studies have focused on the correlation between ocular manifestations of autoimmune diseases and aberrant m6A modification. Zhu et al. [34] reported that m6A expression was significantly increased in extraocular muscle samples from Graves’ ophthalmopathy patients, which suggests that dysregulated m6A regulators may lead to the upregulated expression of genes related to the immune response and inflammatory process, thereby leading to ocular autoimmune diseases. We observed a significant correlation of m6A and METTL3 expression with certain signs of dry eye when assessing tear secretion and ocular surface damage, which indicates that aberrant m6A modification may contribute to the pathogenesis of dry eye in pSS.

However, there are a few limitations of our work. First, our work included only patients from the Department of Ophthalmology, which might lead to selection bias. Further studies could enroll pSS patients from other clinical departments and compare the correlation of m6A expression with other clinical features of pSS. Second, although increased METTL3 and m6A levels influenced the immune response in pSS, the precise regulatory mechanism is unknown. Our results suggest that aberrant m6A modification and METTL3 expression are likely to contribute to pSS pathogenesis, but further in vivo experimental studies are needed. Third, although PBMCs can characterize some disease, the data will be stronger if performed in conjunctiva impression cytology to reflect ocular changes of pSS patients.

Conclusions

In summary, we found an increased level of m6A methylation and elevated expression of MEETL3 in PBMCs from pSS patients with dry eye. Our work revealed that upregulation of m6A and METTL3 was related to the manifestation of serological indicators and dry eye signs in pSS patients with dry eye, which indicates that METTL3 may contribute to the pathogenesis of dry eye related to pSS.

Data Availability

The data used to support the findings of this study are available upon request to the corresponding author.

Abbreviations

pSS:

Primary Sjögren’s syndrome

m6A:

N6-methyladenosine

HCs:

Healthy controls

PBMCs:

Peripheral blood mononuclear cells

Igs:

Immunoglobulins

Cs:

Complement factors

OSDI:

Ocular surface disease index, ST:the Schirmer’s test

CFS:

Corneal fluorescein staining score

TBUT:

Tear break-up time

METTL3:

Methyltransferase-like 3

YTHDF1:

YT521-B homology domains 1

METTL14:

Methyltransferase-like 14

WTAP:

Wilms tumor 1-associated protein

FTO:

Obesity-associated protein

ALKBH5:

Alkylation repair homolog protein 5

YTHDF2:

YT521-B homology domains 2

RA:

Rheumatoid arthritis.

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Acknowledgements

The authors are grateful to all participants and patients for their cooperation in the study and thank American Journal Experts (AJE) for supporting premium editing services for this manuscript.

Funding

This study has received funding by National Natural Science Foundation Project (82070935), the Natural Science Foundation Project of Chongqing (cstc2019jcyj-msxmX0398), Chongqing Medical Scientific Research Project (Joint Project of Chongqing Health Commission and Science and Technology Bureau) (2023GDRC004, 2019MSXM003), and Chongqing Medical University Program for Youth Innovation in Future Medicine (W0185).

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Authors

Contributions

Q Z and Y Z designed the study and supervised the experiment. X Y, X W, TS T, and HP F helped to collect samples and clinical data. J M and XT W performed the experiment and analyzed data. J M and XT W drafted the manuscript. Q Z and Y Z revised the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Yu Zhong or Qi Zhang.

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The authors declare no conflict of interest.

Ethics approval and consent to participate

The research was implemented in accordance with the requirements of the Declaration of Helsinki. All participants provided written informed consent before entering the study. Approval for the present study was obtained from the Ethics Committee of the First Affiliated Hospital of Chongqing Medical University (2020 − 765).

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12886_2023_2988_MOESM1_ESM.docx

Additional file 1: Fig. 1. The correlation analyses of m6A RNA level with the METTL3 mRNA level in the PBMCs of health controls. Fig. 2. Representative raw images showing METTL3 and YTHDF1 expression in the different group

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Ma, J., Wang, X., Yang, X. et al. Increased METTL3 expression and m6A RNA methylation may contribute to the development of dry eye in primary Sjögren’s syndrome. BMC Ophthalmol 23, 252 (2023). https://doi.org/10.1186/s12886-023-02988-0

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