- Case Report
- Open access
- Published:
Spiroplasma infection as a cause of severe congenital keratouveitis, cataract and glaucoma
BMC Ophthalmology volume 24, Article number: 217 (2024)
Abstract
Background
Only seven cases of ocular Spiroplasma infection have been reported to date, all presenting as congenital cataracts with concomitant intraocular inflammation. We describe the first case of Spiroplasma infection initially presenting as a corneal infiltrate.
Case presentation
A 1-month-old girl was referred for a corneal infiltrate in the left eye. She presented in our hospital with unilateral keratouveitis. Examination showed a stromal corneal infiltrate and dense white keratic precipitates in the left eye. Herpetic keratouveitis was suspected and intravenous acyclovir therapy was initiated. Two weeks later, the inflammation in the left eye persisted and was also noticed in the right eye. Acute angle-closure glaucoma and a cataract with dilated iris vessels extending onto the anterior lens capsule developed in the left eye. The inflammation resolved after treatment with azithromycin. Iridectomy, synechiolysis and lensectomy were performed. Bacterial metagenomic sequencing (16 S rRNA) and transmission electron microscopy revealed Spiroplasma ixodetis species in lens aspirates and biopsy. Consequently, a diagnosis of bilateral Spiroplasma uveitis was made.
Conclusions
In cases of congenital cataract with concomitant intraocular inflammation, Spiroplasma infection should be considered. The purpose of this case report is to raise awareness of congenital Spiroplasma infection as a cause of severe keratouveitis, cataract and angle-closure glaucoma in newborns. Performing molecular testing on lens aspirates is essential to confirm diagnosis. Systemic macrolides are suggested as the mainstay of treatment.
Background
Spiroplasma is a genus of bacteria classified within the class Mollicutes, characterized by the lack of a cell wall. Until now, only seven cases of ocular Spiroplasma infection have been described, all presenting as congenital cataracts with concomitant intraocular inflammation. We add one more case to raise awareness of congenital Spiroplasma infection with ophthalmic complications. It is the first report of a Spiroplasma infection presenting as a corneal infiltrate, with rapid evolution towards bilateral anterior uveitis and cataract.
Case presentation
A 1-month-old infant was referred for a corneal infiltrate in the left eye. She was a healthy term infant. Her parents had already noticed blurring of the left eye from the age of 1 week. In retrospect, a C-shaped white corneal infiltrate was visible in the parent’s photographs (Fig. 1A). Slit lamp examination showed central corneal epitheliopathy, stromal oedema and white endothelial precipitates in the left eye. Herpetic keratouveitis in the left eye was suspected. She was admitted to a primary care hospital for intravenous acyclovir administration. Serologic results for Toxoplasma gondii (IgA, IgM, IgG), Toxocara (IgG), Leptospira (combined IgM/IgG), syphilis (treponemal and non-treponemal test), zika virus (IgM, IgG), cytomegalovirus (CMV) (IgM, IgG), herpes simplex virus (HSV) (IgM, IgG), varicella zoster virus (VZV) (IgM, IgG) and human immunodeficiency virus (p24 antigen and HIV-1 and HIV-2 antibodies) were negative.
Despite treatment, 2 weeks later, the girl developed angle-closure glaucoma and buphthalmia with increased keratic precipitates in the left eye and a prepupillary membrane in the right eye (Fig. 1B and C). Acyclovir was stopped and she was admitted to our hospital for further investigation. Treatment with topical prednisolone 1%, dorzolamide/timolol, latanoprost and tropicamide was initiated. Systemic intraocular pressure (IOP) lowering therapy was administered.
Examination under general anaesthesia revealed bilateral anterior uveitis with posterior synechiae and cataract. In the left eye, there was 360° of iridocorneal apposition (Fig. 2) and a white cataract with dilated iris vessels extending from the pupillary margin onto the anterior lens capsule. The fundus was not visible in either eye. The IOP of the left eye was 25 mmHg (right eye 13.5 mmHg). Buphthalmos of the left eye was confirmed by ultrasound imaging. The axial length of the left eye was 21 mm (right eye 18 mm). The corneal diameter of the left eye was 12 mm (right eye 9.5 mm). The infant underwent a bilateral anterior chamber tap and a posterior synechiolysis. In addition, an iridectomy was performed to resolve the pupillary block in the left eye. Cefuroxime was injected intracamerally. Empirical broad spectrum antimicrobial therapy was initiated with oral trimethoprim/sulfamethoxazole and azithromycin. Intravenous prednisolone (2 mg/kg/d for 3 days) was added to the therapeutic regimen.
Inflammation decreased after one week of treatment (Fig. 1D). A lensectomy was performed sequentially in both eyes (left, then right, approximately two months apart). Given the presence of vascularization onto the lens capsule, intravitreal anti-VEGF was administered. Intraoperatively, a chorioretinal lesion in the posterior pole of the left eye was observed.
Polymerase chain reaction (PCR) assays done on anterior chamber fluid were negative for CMV, HSV-1, HSV-2, VZV, Epstein-Barr virus, human herpesvirus 6 and 7. A panbacterial 16 S ribosomal RNA PCR on anterior chamber fluid was negative. Transmission electron microscopy of lens biopsy sections of the left eye showed the presence of Spiroplasma species (Fig. 3). Metagenomic sequencing targeting the complete 16 S rRNA gene performed on aspirates of the right lens identified Spiroplasma ixodetis.
Postoperatively, the infant received topical tobramycin, prednisolone 1%, dorzolamide/timolol and latanoprost. Treatment with oral azithromycin was continued for a total of 16 weeks. Intraocular inflammation did not recur postoperatively. Six weeks after cataract surgery, we observed repeated episodes of IOP rise in the left eye considered to be caused by anterior synechiae. The IOP normalized after laser cyclophotocoagulation. Topical timolol, dorzolamide and latanoprost were continued. At last follow up, 5 months after lensectomy in the left eye and 3.5 months after lensectomy in the right eye, IOP was 17mmHg in the right eye and 20mmHg in the left eye. Binocular visual acuity with aphakic spectacles was 2.4 cycles per cm (Teller Acuity Cards at 38 cm).
Discussion
Spiroplasma is a genus of bacteria belonging to the class Mollicutes. Mollicutes lack a peptidoglycan cell wall and are enclosed in a three-layered cell membrane. These organisms utilize cytoskeletal elements for cell elongation, cell division and motility. Spiroplasma species are helical or spiral-shaped and have a broad host range including plants, insects, and even vertebrates. Spiroplasma have also been shown capable of infecting humans. Still, little is known about the transmission between animals and humans [1]. To date, systemic Spiroplasma infection has been reported in four immunocompromised adult patients and one immunocompetent patient [2,3,4,5]. These patients presented with arthritis, fever, myalgia, fatigue or headaches.
Lorenz et al. were the first to report a case of ocular Spiroplasma species infection in a premature baby, which manifested as a unilateral cataract associated with anterior uveitis [6]. To date, only six more cases of ocular Spiroplasma have been reported worldwide [7,8,9]. The timing of symptom presentation varied, with some infants showing symptoms shortly after birth and others only after a few weeks. All cases involved cataract and anterior uveitis with keratic precipitates and posterior synechiae. However, the severity and specific manifestations were diverse. In most infants, immature dilated iris vessels were present [6,7,8]. Two cases mentioned elevated IOP and buphthalmos [8, 9], similar to our case. Only one patient showed the presence of vitreous inflammation and retinal lesions [8] mirroring our case. In contrast to all the existing reports on ocular Spiroplasma infection, the patient in our study was initially referred for a unilateral corneal infiltrate. A rapid progression to bilateral anterior uveitis and cataract was seen.
The choice of treatment depended on the specific symptoms and findings in each case. Lensectomy, performed in all cases, allowed for the identification of Spiroplasma species. Oral macrolides (josamycin/erythromycin/azithromycin) combined with topical and/or systemic steroids resulted in rapid resolution of inflammation in all patients. Treatment with oral antibiotics was continued for a total of 10 days to 5 weeks. In our case, inflammation decreased after one week of treatment with oral azithromycin. However, the therapy was continued for a total of 16 weeks due to a two-month period between the lensectomy of the left and right eye respectively.
Additional treatment was required in the presence of elevated IOP. In one infant, topical steroids and β-blockers in combination with oral josamycin allowed for the normalization of IOP within a day and for the resolution of inflammation within 2 weeks [9]. In the other case with reported IOP rise, a 5-week course of a topical and systemic anti-inflammatory, antibiotic and IOP-lowering therapeutic regimen was administered [8]. Additionally, in our case, the infant required laser cyclophotocoagulation after iridectomy and lensectomy to normalize IOP.
Conclusion
We present the first case of ocular Spiroplasma infection initially presenting as a corneal infiltrate mimicking herpetic keratouveitis. The purpose of this case report is to raise awareness of congenital Spiroplasma infection as a cause of severe keratouveitis, cataract and angle-closure glaucoma in newborns. Performing bacterial metagenomic sequencing on lens aspirates is essential to confirm diagnosis. Systemic macrolides are suggested as the mainstay of treatment. Further research is needed to develop treatment guidelines.
Data availability
The data supporting our findings is presented within the manuscript.
Abbreviations
- IOP:
-
intraocular pressure
- PCR:
-
polymerase chain reaction
- CMV:
-
cytomegalovirus
- HSV:
-
herpes simplex virus
- VZV:
-
varicella zoster virus
References
Harne S, Gayathri P, Béven L. Exploring Spiroplasma Biology: opportunities and challenges. Front Microbiol. 2020;11:589279.
Aquilino A, Masiá M, López P, Galiana AJ, Tovar J, Andrés M, et al. First human systemic infection caused by Spiroplasma. J Clin Microbiol. 2015;53(2):719–21.
Etienne N, Bret L, Le Brun C, Lecuyer H, Moraly J, Lanternier F, et al. Disseminated Spiroplasma apis infection in patient with agammaglobulinemia, France. Emerg Infect Dis. 2018;24(12):2382–6. https://doi.org/10.3201/eid2412.180567
Mueller NJ, Tini GM, Weber A, Gaspert A, Husmann L, Bloemberg G, et al. Hepatitis from Spiroplasma sp. in an immunocompromised patient. Am J Transpl. 2015;15(9):2511–6. https://doi.org/10.1111/ajt.13254
Eimer J, Fernström L, Rohlén L, Grankvist A, Loo K, Nyman E, et al. Spiroplasma ixodetis infections in Immunocompetent and immunosuppressed patients after Tick exposure, Sweden. Emerg Infect Dis. 2022;28(8):1681–5. https://doi.org/10.3201/eid2808.212524
Lorenz B, Schroeder J, Reischl U. First evidence of an endogenous Spiroplasma sp. infection in humans manifesting as unilateral cataract associated with anterior uveitis in a premature baby. Graefes Arch Clin Exp Ophthalmol. 2002;240(5):348–53.
Matet A, Le Flèche-Matéos A, Doz F, Dureau P, Cassoux N. Ocular Spiroplasma ixodetis in newborns, France. Emerg Infect Dis. 2020;26(2):340–4.
Farassat N, Reich M, Serr A, Küchlin S, Erwemi M, Auw-Hädrich C, et al. Spiroplasma species as a rare cause of congenital cataract and uveitis: a case series. BMC Ophthalmol. 2021;21:434. https://doi.org/10.1186/s12886-021-02201-0
Martin GC, Denier M, Le Fleche-Mateos A. Spiroplasma-induced Uveitis Mimicking Congenital Glaucoma Newborn. 2023;130(3):255.
Acknowledgements
The authors would like to thank Caroline Van Cauwenbergh (PhD, Ophthalmic Clinical Trial Unit, Ghent University Hospital, Belgium) for her help with figure formatting.
Funding
The manuscript was not funded.
Author information
Authors and Affiliations
Contributions
HVH, DR, IJ, HS and PS examined and treated the patient. FN performed the lensectomy. HVH wrote the article and DR and IJ revised it critically for important intellectual content. HH, SV and JB performed the metagenomic sequencing of lens aspirates. LV, JVC, WVDB and LC performed the transmission electron microscopy. All authors approved the final manuscript.
Corresponding author
Ethics declarations
Ethics approval and consent to participate
Not applicable.
Consent for publication
Written informed consent was obtained from the parents of the patient for publication of this case report.
Competing interests
The authors have no competing interests.
Additional information
Publisher’s Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Rights and permissions
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
About this article
Cite this article
Van Haecke, H., Roels, D., Nerinckx, F. et al. Spiroplasma infection as a cause of severe congenital keratouveitis, cataract and glaucoma. BMC Ophthalmol 24, 217 (2024). https://doi.org/10.1186/s12886-024-03480-z
Received:
Accepted:
Published:
DOI: https://doi.org/10.1186/s12886-024-03480-z