- Case report
- Open Access
- Open Peer Review
MAPK activation in mature cataract associated with Noonan syndrome
BMC Ophthalmologyvolume 13, Article number: 70 (2013)
Noonan syndrome is an autosomal, dominantly inherited disease; it is physically characterized by short stature, short neck, webbed neck, abnormal auricles, high arched palate, and cardiovascular malformation. Its pathological condition is thought to be due to a gain-of-function mutation in the Ras-mitogen-activated protein kinase (MAPK) signal transduction pathway. Eyelid abnormalities such as ocular hypertelorism and blepharoptosis are the most commonly observed eye complications.
We report a case of Noonan syndrome associated with mature cataract that required operation. A 42-year-old man was diagnosed with Noonan syndrome at the age of 1 year. He underwent an eye examination after complaining of decreased visual acuity in the right eye and was diagnosed with mature cataract, which was treated by cataract surgery. There were no intraoperative complications, and the postoperative course was uneventful. Protein analysis of lens capsule and epithelium at capsulorhexis showed MAPK cascade proteins such as ERK and p38MAPK were upregulated. An abnormality in the PTPN11 gene was also observed; a potential mechanism of cataract onset may be that opacity of the lens rapidly progressed due to abnormal activation of the Ras-MAPK signal transduction pathway.
This case highlights the possible association of cataract formation with MAPK cascade protein upregulation in Noonan syndrome.
Noonan syndrome (NS, OMIM 163950) is a common genetic disorder characterized by congenital heart disease, short stature, thoracic abnormality, cryptorchidism, mental retardation, and a typical facial appearance, among other characteristics [1, 2]. NS is transmitted as an autosomal dominant trait, and is genetically heterogeneous. Originally, Tartaglia et al. identified missense mutation in PTPN11, which encode Src homology 2-domain phosphatase 2 (SHP-2) in 50% of individuals with NS . Gain-of-function mutations in the PTPN11 gene lead to activation of the Ras-mitogen-activated protein kinase (MAPK) signal transduction pathway. Loss-of function or dominant negative mutations in PTPN11 have been also documented in individual in NS with multiple lentigines (NS/ML, referred to as LEOPARD syndrome (LS; OMIM 151100)) . So far, heterozygous mutations in nine genes (PTPN11, KRAS, NRAS, SOS1, RAF1, BRAF, SHOC2, MEK1, and CBL) in the Ras-MAPK signaling pathway cause NS or closely related conditions, comprising LS, Noonan-like syndrome with loose anagen hair (NS/LAH, OMIM 607721), and the recently documented “CBL mutation associated” syndrome [5, 6]. Noonan-like syndromes comprising Costello syndrome (CS; OMIM 218040), cardiofaciocutaneous syndrome (CFCS, OMIM 115150), neurofibromatosis type 1 (NF1; OMIM 162200), and Legius syndrome (NFLS, OMIM 611431) are disorders clinically related to NS and also harbor mutations in HRAS, KRAS,NF1, BRAF, SPRED1, MEK1, and MEK2 genes [5, 6]. These genes are involved in several developmental processes that control morphology determination, organogenesis, synaptic plasticity, and growth [3–7]. Based on this shared pathogenetic mechanism and clinical overlap, these diseases have been grouped into a single family, which has been termed the Ras-MAPK syndrome, alternatively the RAS-opathies.
Ocular hypertelorism, blepharoptosis, strabismus, ametropia, and cataract have been reported as eye complications in NS . However, the mechanisms of onset of cataract in this order are not clear, and only one case requiring operation has been reported previously . We reported a case of NS associated with mature cataract that required operation. We could also prove MAPK activation and suggested the possible association of MAPK cascade with cataract formations in this disease.
Materials and methods
The Institutional Review Board of the Osaka University Medical School approved the research protocol, and all patients provided informed consent. This study was registered with the approval of Institutional Review Board of the Osaka University Medical School. In the protein analysis, the lens capsule and epithelium at capsulorhexis of the patient with Noonan syndrome or those of senile cataract patient (as control) were collected during surgery.
Western blot analysis of p38α MAP Kinase and ERK phosphorylation
After centrifuge, the sediment homogenized in a blender taken in RIPA buffer (R0278, Sigma-Aldrich, Tokyo, Japan) supplemented with 1% Protease inhibitor cocktail (P8340, Sigma-Aldrich) at 4°C. Lysates were placed on ice for 15 minutes, and centrifuged at 14,000 rpm for 10 minutes at 4°C. The supernatants were collected and preserved at -70°C. Protein concentrations were determined by Coomassie Bradford Protein Assay Kit (Catalog No. 23200, Thermo Fisher Scientific Inc., IL, USA). 13 μg of the total protein per sample was diluted with Laemmli Sample Buffer (Catalog No.161-0737, Bio-Rad, CA, USA), heated at 95°C for 4 min, separated by SDS-PAGE (Multigel II Mini, Cosmo Bio, Tokyo, Japan), and electroblotted onto polyvinylidene fluoride membrane (PVDF, GE Healthcare, Buckinghamshire, UK). After blocking with 2.5% skim milk for 1 hour at room temperature, the membranes were incubated with a p38α MAP Kinase rabbit monoclonal antibody (1:1000, Catalog No.2371, Cell Signaling, Danvers, MA, USA), a rabbit polyclonal anti-phospho-extracellular signal-regulated kinase (ERK) antibody (1:2000, Catalog No.4370, Cell Signaling), a rabbit polyclonal anti-ERK to detect total ERK protein (1:1000, Catalog No.4695, Cell Signaling), or anti-GAPDH (14C10) (1:2000, Catalog No.2118, Cell Signaling) over night at 4°C. After washing with 0.1% Tris-buffered saline (TBS)-Tween, blots were incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG (1:2500, Catalog No.7074, Cell Signaling) for 1 h at room temperature. The blots were then washed three times with 0.1% TBS-Tween and the signals were visualized by an ECL kit (GE Healthcare, Buckinghamshire, UK) according to the manufacturer’s protocol. The densities of immunoreactive bands were measured using Image J for Windows (NIH, Bethesda, MD, USA).
A 42-year-old man experienced decreased visual acuity in the right eye before 2 years, but did not seek treatment. Later, he became aware of severely decreased visual acuity in the right eye several months ago and visited our hospital. The patient was born at 37 weeks of gestation with a weight of 2,300 g and had neonatal asphyxia at birth and had severe jaundice in neonatal period. The patient has a high arched palate, webbed neck, short neck, ocular hypertelorism, exophthalmos, and dilated cardiomyopathy. These factors led to a clinical diagnosis of NS at the age of 1 year (Figure 1). Cardiac disease is controlled through the use of oral drugs. Without receiving growth hormone treatments, his final body height was 154 cm, which is below the potential average height of Japanese men. Initial eye examination suggested that his corrected visual acuity was 0.06 in the right eye and 0.9 in the left with normal intraocular pressure. His corneas and irises showed no abnormalities and no anterior chamber inflammation was observed. Both eyes showed cataracts, and mature cataract was observed in the right eye (Figure 2A, B). The patient had no history of traumatic eye injuries or self-injurious behavior. The left eye fundus showed no abnormal findings (Figure 3A, B). Although the right eye fundus could not be seen, B-mode ultrasonography showed no abnormal findings. No abnormal pupillary light reflexes were observed. In addition to the mature cataract in the right eye, the cataract in the left eye progressed rapidly during the follow-up period, leading to decreased visual acuity. Cataract surgery was indicated for both eyes, and it was performed with no intraoperative complications (Figure 2C, D). He experienced no postoperative intraocular pressure elevation, infection, or delayed inflammation and there were no postoperative abnormalities in the right eye fundus (Figure 3C, D). Genetic analysis revealed a mutation in the PTPN11 gene. The identified PTPN11 mutations (c.188A > G [p.Tyr63Cys]) encode alterations located in the N-terminus of SH2 (Src homology 2) domain, catalytic domain in PTPN11. Protein analysis also showed that the expression of p38α MAP kinase was detected in lens lysates from the patient with NS but, the expression of p38α MAP kinase could not be detected in patient with senile cataract (control), (Figure 4). And also, the p-ERK1/2 expressed in a very low level in lens lysates from the patient with senile cataract (control), while in the patient with NS, it was significantly increased (n = 6, P <0.001) (Figure 5). His postoperative corrected visual acuity was 1.2 in the right eye and 1.0 in the left, and the postoperative course was uneventful.
NS shows physical characteristics similar to those of Turner syndrome, but occurs in both men and women with normal karyotypes and is an independent disease entity from Turner syndrome. Because NS has no explicit diagnostic criteria, it is diagnosed on the basis of its clinical manifestations by using a scoring system . van der Burgt score is usually used for the diagnosis of NS. This scoring system contains 6 categories with 2 alternatives in each category . The syndrome is systemically characterized by short stature, short neck, webbed neck, auricular abnormalities, high arched palate, and cardiovascular malformation, among others. Our patient showed nearly all systemic abnormalities and was diagnosed with definite NS during his childhood. Although ocular hypertelorism, epicanthus, thick eyelids, palpebral ptosis, and strabismus are the primary eye symptoms observed in NS , there has been only a report of cataract complications required operation . Thus, we reported, for the first time, local activation of MAPK signaling pathway in lens of NS patient.
The MAPK family consists of three subfamilies, such as extracellular signal-regulated kinase (ERK) 1 and 2, the c-Jun N-terminal kinase (JNK, also known as the stress-activated protein kinase), and p38 MAPK. The classic MAPKs, ERKs, are principally activated in response to growth factors, whereas JNK and p38 are activated by various stresses, including TNF-α treatment, UV light, x-ray irradiation, heat-shock, and H2O2 treatment . According to the previous reports, transforming growth factor-β (TGF-β) and basic fibroblast growth factor are crucial factors for lens physiology [13–15]. Binding of these factors to lens cell surface receptors initiate cell signaling pathways that include MAPK/ERK signal transduction pathway. The RAS/RAF/MEK/ERK pathway is the classical RAS-MAPK signaling pathway implicated in growth-factor mediated cell proliferation, differentiation, and death . In normal state, appropriate expression of TGF-β is important for ERK activation and physiological cataract formation. The Ras-MAPK pathway is reportedly involved in lens differentiation and maturation [17–19].
We proved MAPK activation in the lens and suggested the possible association of MAPK signaling cascade with cataract formation in cataract with NS. Lee NB et al. reported cataract occurs in 8% of NS . In a study of congenital heart disease, 3 cases (1.3%) out of 240 had NS, and congenital cataract was present in six cases (2.5%) , suggesting that the RAS-opathies, a group of disorders with RAS-MAPK signaling pathway and overlapping neuro-cardio-facial-cutaneous phenotypes, play roles in the possible pathogenesis of this disease. It is well known that Down syndrome and Turner syndrome cause cataract as an eye complication. In contrast, NS shows an overwhelmingly high incidence of corneal and eyelid abnormalities; although cataract complications are observed in this disease, the incidence is lower than that found in Down syndrome . Considering the involvement of MAPK signaling pathway for the cataract formation, genetic analysis revealed a mutation of PTPN11 gene in our patient. The PTPN11 gene is located in the upstream portion of the Ras-MAPK pathway and is thought to be involved in cell growth, differentiation, and senescence [3, 21]. The cataract may have been observed during childhood, but lens opacity may have increased in severity with aging and accumulation of metabolic abnormalities, since no abnormalities were observed in other pathways. It is likely that gain of function of MAPK signaling pathway in NS patients directly activated ERK irrespective of TGF-β upregulation and led to advanced cataract formation.
We report a case of mature cataract complicated by NS and found the local activation of MAPK signaling pathway for cataract formation. Although lens opacity varies in severity, it can progress during the course of the syndrome. The current case highlights the fact that periodic observations of the lens opacities are necessary in patient with NS.
Written consent was obtained from the patient for publication of this material. A copy of the consent is available for review.
Noonan JA, Ehmeke DA: Associated non-cardiac malformatons in children with congenital heart disease. J Paediatr. 1963, 63: 468-470.
Allanson JE: Noonan syndrome. Am J Med Genet C: Semin Med Genet. 2007, 145C: 274-279. 10.1002/ajmg.c.30138.
Tartaglia M, Mehler EL, Goldberg R, Zampino G, Brunner HG, Kremer H, van der Burgt I, Crosby AH, Ion A, Jeffery S, Kalidas K, Patton MA, Kucherlapati RS, Gelb BD: Mutations in PTPN11, encoding the protein tyrosine phosphatase SHP-2, cause Noonan syndrome. Nat Genet. 2001, 29: 465-468. 10.1038/ng772.
Digilio MC, Conti E, Sarkozy A, Mingarelli R, Dottorini T, Marino B, Pizzuti A, Dallapiccola B: Grouping of multiple-lentigines/LEOPARD and Noonan syndromes on the PTPN11 gene. Am J Hum Genet. 2002, 71: 389-394. 10.1086/341528.
Tartaglia M, Gelb BD, Zenker M: Noonan syndrome and clinically related disorders. Best Pract Res Clin Endocrinol Metab. 2011, 25: 161-179. 10.1016/j.beem.2010.09.002.
Roberts AE, Allanson JE, Tartaglia M, Gelb BD: Noonan syndrome. Lancet. 2013, 381: 333-342. 10.1016/S0140-6736(12)61023-X.
Aoki Y, Niihori T, Narumi Y, Kure S, Matsubara Y: The RAS/MAPK syndromes: novel roles of the RAS pathway in human genetic disorders. Hum Mutat. 2008, 29: 992-1006. 10.1002/humu.20748.
Lee NB, Kelly L, Sharland M: Ocular manifestation of Noonan syndrome. Eye (Lond). 1992, 6: 328-334. 10.1038/eye.1992.66.
Bastion ML, Qader AM: Surgical management of unilateral rhegmatogenous retinal detachment associated with ocular coloboma in a 7-year-old child with Noonan syndrome. BMJ Case Rep. 2011, doi:10.1136/bcr.02.2011.3889
Duncan WJ, Fowler RS, Farkas LG, Ross RB, Wright AW, Bloom KR, et al: A comprehensive scoring system for elevation Noonan syndrome. Am J Med Genet. 1981, 10: 37-50. 10.1002/ajmg.1320100106.
van der Burgt I, Berends E, Lommen E, van Beersum S, Hamel B, Mariman E: Clinical and molecular studies in a large Dutch family with Noonan syndrome. Am J Med Genet. 1994, 53: 187-191. 10.1002/ajmg.1320530213.
Chang L, Karin M: Mammalian MAP kinase signaling cascades. Nature. 2001, 410: 37-40. 10.1038/35065000.
Ibaraki N, Lin LR, Reddy VN: Effects of growth factors on proliferation and differentiation in human lens epithelial cells in early subculture. Invest Ophthalmol Vis Sci. 1995, 36: 2304-2312.
Kampmeier J, Baldysiak-Figiel A, de Jong-Hesse Y, Lang GK, Lang GE: Effect of growth factors on proliferation and expression of growth factor receptors in a human lens epithelial cell line. J Cataract Refract Surg. 2006, 32: 510-514. 10.1016/j.jcrs.2005.08.063.
Schneider R, Jensen SA, Whiteman P, McCullagh JS, Redfield C, Handford PA: Biophysical characterization of fibulin-5 proteins associated with disease. J Mol Biol. 2010, 401: 605-617. 10.1016/j.jmb.2010.06.039.
Malumbres M, Barbacid M: RAS oncogenes: the first 30 years. Nat Rev Cancer. 2003, 3: 459-465. 10.1038/nrc1097.
Martinez G, de Iongh RU: The lens epithelium in ocular health and disease. Int J Biochem Cell Biol. 2010, 42: 1945-1963. 10.1016/j.biocel.2010.09.012.
Zhang P, Xing K, Randazzo J, Blessing K, Lou MF, Kador PF: Osmotic stress, not aldose reductase activity, directly induces growth factors and MAPK signaling changes during sugar cataract formation. Exp Eye Res. 2012, 101: 36-43.
Shakespeare TI, Sellitto C, Li L, Rubinos C, Gong X, Srinivas M, et al: Interaction between Connexin50 and mitogen-activated protein kinase signaling in lens homeostasis. Mol Biol Cell. 2009, 20: 2582-2592. 10.1091/mbc.E08-12-1257.
Mansour AM, Bitar FF, Traboulsi EI, Kassak KM, Obeid MY, Megarbane A, Salti HI: Ocular pathology in congenital heart disease. Eye (Lond). 2005, 19: 29-34. 10.1038/sj.eye.6701408.
Jorge AA, Malaquias AC, Arnhold IJ, Mendonca BB: Noonan syndrome and related disorders: a review of clinical features and mutations in genes of the RAS/MAPK pathway. Horm Res. 2009, 71: 185-193. 10.1159/000201106.
The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1471-2415/13/70/prepub
No financial support was received for this submission.
The authors have no proprietary interest in any aspect of this report.
The authors declare that they have no competing interests.
NH: patient interaction and diagnosis, Western blot experiment, drafting of manuscript, final approval of manuscript, XP: patient diagnosis, Western blots experiment, final approval of manuscript, KN: patient interaction and diagnosis, final approval of manuscript. All authors read and approved the final manuscript.